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Tumor Biology

, Volume 34, Issue 4, pp 2141–2146 | Cite as

Clinicopathological significance of BTF3 expression in colorectal cancer

  • Chao-Jie Wang
  • Hanna Frånbergh-Karlson
  • Da-Wei Wang
  • Gunnar Arbman
  • Hong Zhang
  • Xiao-Feng Sun
Research Article

Abstract

Basic transcription factor 3 (BTF3) is a general RNA polymerase II transcription factor and is also involved in apoptosis regulation. Increasing evidence shows that BTF3 is aberrantly expressed in several kinds of malignancies, but there is no study to analyze BTF3 expression in colorectal cancer (CRC) patients. Applying immunohistochemistry, we detected BTF3 in CRCs (n = 156), the corresponding distant (n = 42), adjacent normal mucosa (n = 96), lymph node metastases (n = 35), and analyzed its relationships with clinicopathological and biological variables. Our results showed that BTF3 staining significantly increased from distant or adjacent normal mucosa to primary CRCs (p < 0.0001) or metastases (p = 0.002 and p < 0.0001). BTF3 was higher in distal cancers than in proximal cancers (57 % vs. 39 %, p = 0.041). It also showed stronger staining in primary CRCs stage I and II than that in stage III and IV (64 % vs. 35 %, p = 0.0004), or metastases (64 % vs. 29 %, p = 0.004). Cancers with better differentiation had a higher expression than those with worse differentiation (56 % vs. 37 %, p = 0.031). There were positive correlations of BTF3 expression with nuclear factor kappa B (NF-κB), RAD50, MRE11, NBS1, and AEG-1 (p < 0.05). In conclusion, BTF3 overexpression may be an early event in CRC development and could be useful biomarker for the early stage of CRCs. BTF3 has positive correlations with NF-κB, RAD50, MRE11, NBS1 and AEG-1, and might influence complex signal pathways in CRC.

Keywords

Basic transcription factor 3 Biomarker Colorectal cancer Immunohistochemistry 

Notes

Acknowledgments

This study was supported by grants from the Swedish Cancer Foundation, Swedish Research Council and the Health Research Council in the southeast of Sweden.

Conflicts of interest

None

Supplementary material

13277_2013_745_MOESM1_ESM.tif (5.5 mb)
ESM 1 (TIFF 5593 kb)

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2013

Authors and Affiliations

  • Chao-Jie Wang
    • 1
    • 2
  • Hanna Frånbergh-Karlson
    • 2
  • Da-Wei Wang
    • 3
  • Gunnar Arbman
    • 4
  • Hong Zhang
    • 5
  • Xiao-Feng Sun
    • 6
  1. 1.Department of OncologyHenan Provincial People’s Hospital and People’s Hospital of Zhengzhou UniversityZhengzhouChina
  2. 2.Faculty of Health Sciences, Division of Oncology, Department of Clinical and Experimental MedicineUniversity of LinköpingLinköpingSweden
  3. 3.Department of StomatologyThe Third Hospital of Hebei Medical UniversityShijiazhuangChina
  4. 4.Department of Surgery, Vrinnevi HospitalUniversity of LinköpingNorrköpingSweden
  5. 5.School of MedicineÖrebro UniversityÖrebroSweden
  6. 6.Faculty of Health Sciences, Division of Oncology, Department of Clinical and Experimental Medicine, County Council of ÖstergötlandUniversity of LinköpingLinköpingSweden

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