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Tumor Biology

, Volume 34, Issue 3, pp 1807–1812 | Cite as

Clinical significance of RUNX2 expression in patients with nonsmall cell lung cancer: a 5-year follow-up study

  • Hong Li
  • Ren-Jie Zhou
  • Guo-Qiang Zhang
  • Jian-Ping Xu
Research Article

Abstract

This study was designed to evaluate expression and prognostic significance of runt-related transcription factor (RUNX)-2 in human nonsmall cell lung cancer (NSCLC). RUNX2 expression was examined at mRNA and protein levels by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot in NSCLC tissues and adjacent normal tissues. In addition, RUNX2 expression was analyzed by immunohistochemistry in 121 clinicopathologically characterized NSCLC cases. The relationship between the expression of RUNX2 and clinicopathological characteristics and prognosis was statistically analyzed. Both qRT-PCR and Western blot demonstrated that RUNX2 mRNA and protein levels were significantly higher in NSCLC tissues compared to the adjacent normal tissues from the same individual. Immunohistochemistry analysis showed that RUNX2 expression was significantly correlated with tumor size, tumor stage, and lymph node metastasis. Higher RUNX2 expression was associated with shorter postoperative survival time of NSCLC patients by Kaplan–Meier method and was found to be an independent risk factor that influences the postoperative survival time of NSCLC patients by Cox regression analysis. In conclusion, these data showed that RUNX2 may play an important role in NSCLC tumorigenesis, and RUNX2 might serve as a novel prognostic marker in NSCLC.

Keywords

RUNX2 NSCLC Prognosis 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 81070094 and No. 30972964).

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2013

Authors and Affiliations

  • Hong Li
    • 1
  • Ren-Jie Zhou
    • 2
  • Guo-Qiang Zhang
    • 3
  • Jian-Ping Xu
    • 4
  1. 1.Department of Anesthesiology, Xinqiao HospitalThird Military Medical UniversityChongqingChina
  2. 2.Department of Emergency, Xinqiao HospitalThird Military Medical UniversityChongqingChina
  3. 3.Department of Thoracic Surgery, Xinqiao HospitalThird Military Medical UniversityChongqingChina
  4. 4.Department of Pathology, Xinqiao HospitalThird Military Medical UniversityChongqingChina

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