Tumor Biology

, Volume 34, Issue 3, pp 1523–1530 | Cite as

Lactate dehydrogenase A is overexpressed in pancreatic cancer and promotes the growth of pancreatic cancer cells

  • Yefei Rong
  • Wenchuan Wu
  • Xiaoling Ni
  • Tiantao Kuang
  • Dayong Jin
  • Dansong WangEmail author
  • Wenhui LouEmail author
Research Article


The prognosis for pancreatic cancer is very poor, and developing new therapeutic strategies for this cancer is needed. Recently, the Warburg effect (aerobic glycolysis) has attracted much attention for its function in the tumorigenesis. Lactate dehydrogenase A (LDHA) executes the final step of aerobic glycolysis and has been reported to be involved in the tumor progression. However, the function of LDHA in pancreatic cancer has not been studied. Here, we found that the expression of LDHA was elevated in the clinical pancreatic cancer samples. Forced expression of LDHA promoted the growth of pancreatic cancer cells, while knocking down the expression of LDHA inhibited cell growth dramatically. Moreover, silencing the expression of LDHA inhibited the tumorigenicity of pancreatic cancer cells in vivo. Mechanistically, knocking down the expression of LDHA activated apoptosis pathway. Taken together, our study revealed the oncogenic role of LDHA in pancreatic cancer and suggested that LDHA might be a potential therapeutic target.


Pancreatic cancer LDHA Cell apoptosis Proliferation 



This work was supported by the Shanghai Science and Technology Fund JC1402200 and the Research Special Fund for Public Welfare Industry of Health 201202007.

Conflicts of interest



  1. 1.
    Jemal A, Siegel R, Ward E, et al. Cancer statistics. CA Cancer J Clin. 2009;59:225–49.PubMedCrossRefGoogle Scholar
  2. 2.
    Jemal A, Siegel R, Ward E, et al. Cancer statistics. CA Cancer J Clin. 2008;58:71–96.PubMedCrossRefGoogle Scholar
  3. 3.
    Sohn TA, Yeo CJ, Cameron JL, et al. Resected adenocarcinoma of the pancreas—616 patients: results, outcomes, and prognostic indicators. J Gastrointest Surg. 2000;4:567–79.PubMedCrossRefGoogle Scholar
  4. 4.
    Lewis BC, Shim H, Li Q, et al. Identification of putative c-Myc-responsive genes: characterization of rcl, a novel growth-related gene. Mol Cell Biol. 1997;17:967–78.Google Scholar
  5. 5.
    Shim H, Dolde C, Lewis BC, et al. C-Myc transactivation of LDH-A: implications for tumor metabolism and growth. Proc Natl Acad Sci USA. 1997;94:6658–63.PubMedCrossRefGoogle Scholar
  6. 6.
    Yao F, Zhao T, Zhong C, et al. LDHA is necessary for the tumorigenicity of esophageal squamous cell carcinoma. Tumor Biol. 2012;34:25–31.CrossRefGoogle Scholar
  7. 7.
    Cai Z, Zhao JS, Li JJ, et al. A combined proteomic and metabolomic profiling of gastric cardia cancer reveals characteristic dysregulations in glucose metabolism. Mol Cell Proteomics. 2010;9:2617–28.PubMedCrossRefGoogle Scholar
  8. 8.
    Warshaw AL, Fernandez-del CC. Pancreatic carcinoma. N Engl J Med. 1992;316:455–65.CrossRefGoogle Scholar
  9. 9.
    Hidalgo M. Pancreatic cancer. N Engl J Med. 1992;362:1605–17.CrossRefGoogle Scholar
  10. 10.
    Hruban RH et al. Progression model for pancreatic cancer. Clin Cancer Res. 2000;6:2969–72.PubMedGoogle Scholar
  11. 11.
    Gatenby RA, Gillies RJ. Why do cancers have high aerobic glycolysis? Nat Rev Cancer. 2004;4:891–9.PubMedCrossRefGoogle Scholar
  12. 12.
    Gillies RJ, Robey I, Gatenby RA. Causes and consequences of increased glucose metabolism of cancers. J Nucl Med. 2008;49:24S–42S.PubMedCrossRefGoogle Scholar
  13. 13.
    Martínez-Zaguilán R, Seftor EA, Seftor RE, et al. Acidic pH enhances the invasive behavior of human melanoma cells. Clin Exp Metastasis. 1996;14:176–86.PubMedCrossRefGoogle Scholar
  14. 14.
    Schlappack OK, Zimmermann A, Hill PR. Glucose starvation and acidosis: effect on experimental metastatic potential, DNA content and MTX resistance of murine tumour cells. Br J Cancer. 1991;64:663–70.PubMedCrossRefGoogle Scholar
  15. 15.
    Dang CV, Semenza GL. Oncogenic alterations of metabolism. Trends Biochem Sci. 1999;24:68–72.PubMedCrossRefGoogle Scholar
  16. 16.
    Sheng SL, Liu JJ, Dai YH, et al. Knockdown of lactate dehydrogenase A suppresses tumor growth and metastasis of human hepatocellular carcinoma. FEBS J. 2012;279:3898–910.PubMedCrossRefGoogle Scholar

Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2013

Authors and Affiliations

  1. 1.Pancreatic Cancer Group, General Surgery Department, Zhongshan HospitalFudan UniversityShanghaiChina

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