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Tumor Biology

, Volume 34, Issue 2, pp 1139–1144 | Cite as

Correlation between Rho-kinase pathway gene expressions and development and progression of glioblastoma multiforme

  • Ibrahim Erkutlu
  • Ahmet Cigiloglu
  • Mehmet Emin Kalender
  • Mehmet Alptekin
  • A. Tuncay Demiryurek
  • Ali Suner
  • Esma Ozkaya
  • Mustafa Ulaslı
  • Celalettin Camcı
Research Article

Abstract

Glioblastoma multiforme (GBM) is the most common and the most aggressive primary malignant tumor of the brain. Prognostic factors in GBM can be sorted as age, tumor localization, tumor diameter, symptom period and type, the extent of surgery, postoperative tumor volume, and adjuvant radiotherapy and/or chemotherapy status. Besides the interactions between actin microfilaments, microtubules, and intermediate filaments, environmental factors and intracellular signals which regulate them affect the cell invasion. Rho proteins and therefore Rho-kinase activation play important role at these changes. The aim of this study is to evaluate the relationship between the Rho-kinase pathway gene expressions and prognosis in GBM. Ninety-eight patients diagnosed as GBM between 2001 and 2010 were enrolled into the study. RNA was obtained from the paraffinized tumor tissue of the patients with formalin-fixed, paraffin-embedded RNA isolation kit and the mRNA expressions of 26 genes were investigated. There was a statistically significant negative correlation between the ages at the diagnosis and survival. There was a significant relationship between the overexpression of Rho-kinase pathway-related genes LIMK1, CFL1, CFL2, and BCL2 and low expression of MAPK1 gene and the survival of the patients. These results demonstrate for the first time that there is a marked contribution of Rho-kinase pathway-related genes to the progression and survival of the GBM. The expression of these genes may be related to response of multimodal therapy or these parameters could be used to determine possible unresponsive patients before treatment.

Keywords

Glioblastoma multiforme Rho-kinase LIM-kinase Cofilin 

Notes

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2013

Authors and Affiliations

  • Ibrahim Erkutlu
    • 1
  • Ahmet Cigiloglu
    • 2
  • Mehmet Emin Kalender
    • 3
    • 6
  • Mehmet Alptekin
    • 1
  • A. Tuncay Demiryurek
    • 4
  • Ali Suner
    • 3
  • Esma Ozkaya
    • 5
  • Mustafa Ulaslı
    • 5
  • Celalettin Camcı
    • 3
  1. 1.Department of Neurosurgery, School of MedicineGaziantep UniversityGaziantepTurkey
  2. 2.Department of Internal Medicine, School of MedicineGaziantep UniversityGaziantepTurkey
  3. 3.Division of Medical Oncology, Department of Internal Medicine, School of Medicine, Gaziantep Oncology HospitalGaziantep UniversityGaziantepTurkey
  4. 4.Department of Medical Pharmacology, School of MedicineGaziantep UniversityGaziantepTurkey
  5. 5.Department of Medical Biology, School of MedicineGaziantep UniversityGaziantepTurkey
  6. 6.Department of Medical Oncology, School of Medicine, Gaziantep Oncology HospitalGaziantep UniversityGaziantepTurkey

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