Tumor Biology

, Volume 34, Issue 2, pp 983–986 | Cite as

Characterization of cell-free circulating DNA in plasma in patients with prostate cancer

  • Pâmela Oliveira Delgado
  • Beatriz Costa A. Alves
  • Flávia de Sousa Gehrke
  • Renata Kelly Kuniyoshi
  • Marcelo Langer Wroclavski
  • Auro Del Giglio
  • Fernando Luiz Affonso Fonseca
Research Article


Cell-free circulating DNA in plasma and serum may serve as a biomarker for malignant tumor detection and follow up in patients with a variety of solid tumors including prostate cancer. In healthy patients, DNA is normally released from an apoptotic source which generates small fragments of cell-free DNA, whereas cancer patients have cell-free circulating DNA that originated from necrosis, autophagy, or mitotic catastrophe. Cell-free circulating DNA levels were measured by a quantitative real-time PCR method with a set of primers targeted to amplify the consensus ALU apoptotic versus necrotic origin. Prostate cancer patients before and 3 months after diagnosis showed cell-free circulating DNA released at apoptotic and non-apoptotic cell death. Interestingly, all patients after 6 months demonstrated DNA released at non-apoptotic cell. The principal source of cell-free circulating DNA is of apoptotic and non-apoptotic cell death. However, during treatment, this feature could change. Therefore, the study of cell-free circulating DNA would be important to follow the evolution of the disease during the treatment.


Prostate Cancer Cell-free circulating DNA DNA integrity ALU sequence 



This work was supported by Fapesp Grant 10/ 50490-6. POD, FSG, and RKK were postgraduate fellows from the Instituto Uniemp/Instituto Israelita de Ensino e Pesquisa Albert Einstein.


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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2012

Authors and Affiliations

  • Pâmela Oliveira Delgado
    • 1
  • Beatriz Costa A. Alves
    • 1
  • Flávia de Sousa Gehrke
    • 1
  • Renata Kelly Kuniyoshi
    • 1
  • Marcelo Langer Wroclavski
    • 2
  • Auro Del Giglio
    • 1
  • Fernando Luiz Affonso Fonseca
    • 1
    • 3
  1. 1.Oncology/Hematology DisciplineFMABCSanto AndréBrazil
  2. 2.Urology DisciplineFMABCSanto AndréBrazil
  3. 3.Biological Science DepartmentUNIFESPDiademaBrazil

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