Effects of manganese superoxide dismutase (MnSOD) expression on regulation of esophageal cancer cell growth and apoptosis in vitro and in nude mice
Manganese superoxide dismutase (MnSOD) catalyzes superoxide radical (O2 −) into hydrogen peroxide (H2O2), which is further catalyzed by the combined action of glutathione peroxidase (GPx) and catalase (CAT) into water and oxygen. MnSOD plays a role in cell protection from superoxide damage. This study aimed to investigate the effects of MnSOD on regulation of esophageal squamous cell carcinoma cell growth, apoptosis, and cell cycle distribution in vitro and tumor formation and growth in nude mouse xenografts. The data showed that differential levels of MnSOD expression had different effects on tumor cell proliferation, apoptosis, plating efficiency (PE), and cell cycle distribution in vitro and tumor formation and growth in nude mice. In particular, high levels of MnSOD expression promoted TE-1 cell growth and PE rate in vitro and in nude mice, whereas moderate MnSOD expression suppressed tumor cell growth and PE rate but induced more cell apoptosis. Thus, these data demonstrated the dual effects of MnSOD protein in esophageal squamous cell carcinoma and further study will confirm these current data.
KeywordsManganese superoxide dismutase Esophageal carcinoma Cell proliferation Transfection
This study was supported, in part, by grants from the National Natural Science Foundation of China (#30,540,005), the Ministry of Personnel for the High-Level Talent Returned from Overseas (#Guo-Ren-Ting-Fa -118), the Starting Fund by the Ministry of Education for Personnel Returning from Abroad (#Jiao-Wai-Si-Liu -101), and Funded Projects on the Featured Disciplines (group) in the Institutions of Higher Learning in Hebei Province (#Ji-Jiao-Gao -52).
Conflict of interest
- 6.Komatsy M, Kuroda M, Wang Y, St Clair D, Urano M, Akaki S, Asaumi J, Kawasaki S, Hiraki Y, Kanazawa S. Manganese superoxide dismutase overexpression changes plating efficiency bidirectionally according to change in redox for SaOS2 human osteosarcoma cell line. Int J Oncol. 2005;26:853–62.Google Scholar
- 7.Wang Y, Komatsy M, Gao XS, Asaumi J, Shibuya K, Kawasaki S, Akaki S, St Clair D, Hiraki Y, Kanazawa S. Hydrogen peroxide overload increases adriamycin-induced apoptosis of SaOS2FM, a manganese superoxide dismutase-overexpressing human osteosarcoma cell line. Int J Oncol. 2005;26:1291–300.PubMedGoogle Scholar
- 9.Epperly MW, Sikora C, Defilippi S, Bray J, Koe G, Liggitt D, Luketich JD, Greenberger JS. Plasmid/liposome transfer of the human manganese superoxide dismutase transgene prevents ionizing irradiation-induced apoptosis in human esophagus organ explant culture. Int J Cancer. 2000;90:128–37.PubMedCrossRefGoogle Scholar
- 21.Chen J, Lin GX, Tang F, et al. Effects of manganese superoxide dismutase transfection on the proliferation of SGC7901 gastric cancer cell. Fudan Univ J Med Sci. 2004;31:19–23.Google Scholar