Tumor Biology

, Volume 34, Issue 2, pp 743–748 | Cite as

Effects of heat stress on the level of heat shock protein 70 on the surface of hepatocellular carcinoma Hep G2 cells: implications for the treatment of tumors

  • Naizhong Cui
  • Yongping Xu
  • Zhenhui Cao
  • Fanxing Xu
  • Peng Zhang
  • Liji Jin
Research Article


The ability to distinguish tumor cells from normal cells is vital to allow the immune system to selectively destroy tumor cells. In order to find an effective marker, we used enzyme-linked immunosorbent assay, immunocytochemistry, immunofluorescence, and flow cytometry to investigate the effects of heat stress on the amount of heat shock protein 70 on the surface of tumor cells (Hep G2 cells). Heat shock protein 70 is the major stress-induced heat shock protein found on the surface of tumor cells. Our results indicate that the percentage of Hep G2 cells with a detectable level of heat shock protein 70 on their cell surface increased significantly (P < 0.05) following heat stress at 42 °C for 2 h (up to 1.92 times the level before heat treatment). The detectable level of heat shock protein 70 on the surface of Hep G2 cells reached its peak 12 h after treatment. However, the fluorescent intensity of stressed and unstressed Hep G2 cells was not significantly different (P > 0.05). The increase in the level of heat shock protein 70 on the surface of tumor cells following heat stress could provide a basis for finding novel immunotoxins as targets for drug action and may have application to be used in conjunction with hyperthermia in the treatment of tumors.


Cell surface proteins Heat stress Heat shock protein 70 Hep G2 cells 



We thank Dr. Alan K Chang (Dalian University of Technology, Dalian, China) for critical discussion and correction of the manuscript.

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2012

Authors and Affiliations

  • Naizhong Cui
    • 1
  • Yongping Xu
    • 1
  • Zhenhui Cao
    • 1
  • Fanxing Xu
    • 1
  • Peng Zhang
    • 2
  • Liji Jin
    • 1
  1. 1.Ministry of Education Center for Food Safety of Animal OriginSchool of Life Science and Biotechnology, Dalian University of TechnologyDalianChina
  2. 2.Liaoning Provincial Key Laboratory of HydrobiologyCollege of Life Science and Technology, Dalian Ocean UniversityDalianChina

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