Tumor Biology

, Volume 34, Issue 3, pp 1391–1397 | Cite as

The effects of Hsp90 expression alteration on spinal metastases of breast carcinoma

  • Wangjun Yan
  • Jianru Xiao
  • Tielong Liu
  • Wending Huang
  • Xinghai Yang
  • Zhipeng Wu
  • Quan Huang
  • Ming Qian
Research Article


Previous studies have demonstrated that Hsp90 is closely associated with tumor metastases, and inhibition of Hsp90 expression can result in reduced tumor invasiveness and migration capability. However, its role in spinal metastases of breast carcinoma remains unknown. The paper aimed to further detect Hsp90 expression in a mouse model of spinal metastases of breast carcinoma which was established by left ventricular injection of breast cancer cell lines TM40D to nude mice. The BALB/c nude mice were divided into four groups at random: blank control group (n = 10), model group (n = 30), negative control group (n = 10), and experimental group (n = 30). Mice in the experimental group were given intraperitoneal injection of 12 mg/kg 17-allylamino-demethoxy geldanamycin (17-AAG), an inhibitor for Hsp90. The protein and mRNA expressions of Hsp90 were respectively determined using immunohistochemistry and real-time PCR. Bioluminescence imaging, dissection, and hematoxylin and eosin staining were performed to observe tumor formation and bone damage. Our results suggested that Hsp90 expression in mice with breast cancer metastasis in the spine was significantly higher than that in normal mice. Furthermore, Hsp90 expression was decreased and the spinal metastasis from breast cancer was inhibited by 17-AAG application. Hsp90 could be considered as an indicator to forecast tumor metastasis and provide a target for the treatment of spinal metastasis of breast cancer.


Spinal metastases Hsp90 17-Allylamino-demethoxy geldanamycin Breast cancer Real-time PCR 


Conflicts of interest

The authors declare that they have no competing interests.


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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2013

Authors and Affiliations

  • Wangjun Yan
    • 1
  • Jianru Xiao
    • 1
  • Tielong Liu
    • 1
  • Wending Huang
    • 1
  • Xinghai Yang
    • 1
  • Zhipeng Wu
    • 1
  • Quan Huang
    • 1
  • Ming Qian
    • 1
  1. 1.Spine Tumor Center, Department of OrthopedicsChangzheng Hospital Affiliated the Second Military Medical UniversityShanghaiChina

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