Tumor Biology

, Volume 33, Issue 6, pp 1899–1905 | Cite as

Negative regulation of cell motile and invasive activities by lysophosphatidic acid receptor-3 in colon cancer HCT116 cells

  • Rie Fukui
  • Eriko Tanabe
  • Misaho Kitayoshi
  • Kyohei Yoshikawa
  • Nobuyuki Fukushima
  • Toshifumi Tsujiuchi
Research Article


Lysophosphatidic acid (LPA) mediates a wide range of biological responses with G protein-coupled transmembrane receptors (LPA receptors). So far, at least six types of LPA receptors (LPA receptor-1 (LPA1) to LPA6) have been identified. Recently, it has been reported that LPA3 indicates opposite effects on cellular functions of cancer cells. In the present study, to assess a biological role of LPA3 on cell migration ability of colon cancer cells, we generated LPA receptor-3 (LPAR3) knockdown (HCT-sh3-3) cells from HCT116 and measured cell motile and invasion activities. In motility assay with a cell culture insert, HCT-sh3-3 cells showed significantly high cell motile activity, compared with control cells. For invasion assay, the filter was coated with Matrigel. The invasive activity of HCT-sh3-3 cells was significantly higher than that of control cells. Furthermore, we also examined the effects of LPAR3 knockdown on the interaction between colon cancer cells and endothelial F-2 cells. When F-2 cells were cultured with serum-free DMEM containing a supernatant from HCT-sh3-3 cells, the cell growth rate and migration activity of F-2 cells were significantly stimulated, associating with the elevated expressions of vascular endothelial growth factor (VEGF)-A and VEGF-C genes in HCT-sh3-3 cells. These results suggest that LPA3 may act as a negative regulator on cell motile and invasive abilities of colon cancer HCT116 cells.


LPA LPA receptor-3 Cell migration Cell invasion Colon cancer 



This study was supported in part by a Grant-in-Aid (24590493) for Scientific Research (C) from Ministry of Education, Culture, Sports, Science and Technology, Japan, grants from the Ministry of Health, Labour and Welfare of Japan, and grants (RK-027) from the Faculty of Science and Engineering, Kinki University.

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2012

Authors and Affiliations

  • Rie Fukui
    • 1
  • Eriko Tanabe
    • 1
  • Misaho Kitayoshi
    • 1
  • Kyohei Yoshikawa
    • 1
  • Nobuyuki Fukushima
    • 2
  • Toshifumi Tsujiuchi
    • 1
  1. 1.Division of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and EngineeringKinki UniversityHigashiosakaJapan
  2. 2.Division of Molecular Neurobiology, Department of Life Science, Faculty of Science and EngineeringKinki UniversityHigashiosakaJapan

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