Tumor Biology

, Volume 33, Issue 4, pp 1209–1214 | Cite as

Evaluation and prognostic significance of human tissue kallikrein-related peptidase 10 (KLK10) in colorectal cancer

  • Constantina Petraki
  • Youssef M. Youssef
  • William Dubinski
  • Zsuzsanna Lichner
  • Andreas Scorilas
  • Maria D. Pasic
  • Vassilios Komborozos
  • Bishoy Khalil
  • Catherine Streutker
  • Eleftherios P. Diamandis
  • George M. Yousef
Research Article


The prognosis of patients with colorectal cancer (CRC) is assessed through conventional clinicopathological parameters, which are not always accurate. Members of the human kallikrein-related peptidases gene family represent potential cancer biomarkers. The aim of this study was to investigate the expression of human tissue kallikrein-related peptidase 10 (KLK10) by immunohistochemistry in CRC, to correlate this expression with various histopathological and clinical variables, and to evaluate its significance as a predictor of disease outcome. KLK10 expression was evaluated by immunohistochemistry and a combined expression score was calculated for each case based on intensity and percentage of positivity. A statistically significant positive association was observed between KLK10 and tumor stage and liver metastases (p = 0.015 and p = 0.035, respectively). Paradoxically, a negative association was observed between KLK10 and tumor grade (p = 0.009). Kaplan–Meier survival curves and univariate analysis showed that both KLK10 expression and stage had statistically significant correlations with disease-free survival (DFS) (p = 0.030 and p < 0.001, respectively) and overall survival (p = 0.010 and p = 0.001, respectively). Cox multivariate analysis showed that both KLK10 expression and stage were independent predictors of unfavorable DFS (p = 0.057 and p = 0.001, respectively) and overall survival (p = 0.009 and p = 0.001, respectively). In conclusion, KLK10 immunostaining is an independent prognostic marker in patients with CRC.


KLK Kallikreins KLK10 Colorectal cancer Prognosis 

Non-standard abbreviations


Colorectal cancer


Proportion score


Intensity score


Total score





This work was supported by grants from the Canadian Cancer Society (CCS grant # 20185), the Ministry of Research and Innovation of the Government of Ontario, the Kidney Foundation of Canada, Prostate Cancer Canada (grant # 2010-555), and the Cancer Research Society.

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2012

Authors and Affiliations

  • Constantina Petraki
    • 1
  • Youssef M. Youssef
    • 2
    • 3
  • William Dubinski
    • 2
    • 3
  • Zsuzsanna Lichner
    • 2
    • 3
  • Andreas Scorilas
    • 4
  • Maria D. Pasic
    • 2
  • Vassilios Komborozos
    • 5
  • Bishoy Khalil
    • 3
  • Catherine Streutker
    • 2
    • 3
  • Eleftherios P. Diamandis
    • 2
  • George M. Yousef
    • 2
    • 3
  1. 1.Departments of PathologyMetropolitan HospitalAthensGreece
  2. 2.Department of Laboratory Medicine and PathobiologyUniversity of TorontoTorontoCanada
  3. 3.Department of Laboratory Medicine, and the Keenan Research Centre in the Li Ka Shing Knowledge InstituteSt. Michael’s Hospital TorontoTorontoCanada
  4. 4.Department of Biochemistry and Molecular BiologyFaculty of Biology, University of AthensAthensGreece
  5. 5.Department of General SurgeryEvangelismos HospitalAthensGreece

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