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Tumor Biology

, Volume 33, Issue 5, pp 1281–1290 | Cite as

Differentially expressed proteins identified in overexpressed let-7a gastric carcinoma cells by two-dimensional polyacrylamide gel electrophoresis

  • Yimin Zhu
  • Xingyuan Xiao
  • Lairong Dong
  • Zhiming Liu
Research Article

Abstract

MicroRNAs are small noncoding RNA molecules that control the expression of target genes. Our previous studies show that let-7a decreased in gastric carcinoma and that up-regulation of let-7a by gene augmentation inhibited gastric carcinoma cell growth both in vitro and in vivo, whereas it remains largely unclear as to how let-7a affects tumor growth. In this study, proteins associated with the function of let-7a were detected in high-throughput screening. The cell line of SGC-7901 stably overexpressing let-7a was successfully established by gene clone. Two-dimensional gel electrophoresis (2-DE) was used to separate the total proteins of SGC-7901/let-7a, SGC-7901/EV and SGC-7901, and PDQuest software was applied to analyze 2-DE images. Ten differential protein spots were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and they may be the proteins associated with let-7a function. The overexpressed proteins include antioxidant protein 2, insulin-like growth factor binding protein 2, protein disulfide isomerase A2, C-1-tetrahydrofolate synthase, cyclin-dependent kinase inhibitor1 (CDKN1) and Rho-GTPase activating protein 4. The underexpressed proteins consisted of S-phase kinase-associated protein 2 (Spk2), platelet membrane glycoprotein, fibronectin and Cks1 protein. Furthermore, the different expression levels of the partial proteins (CDKN1,Spk2 and Fibronectin) were confirmed by Western blot analysis. The data suggest that these differential proteins are involved in novel let-7a signal pathway, and these findings provided the basis to comprehensively investigate the functional mechanisms of let-7a in gastric carcinoma.

Keywords

Proteomics miRNA Gastric carcinoma 

Notes

Acknowledgements

This work was supported by research grants from the key science and technology projects of Guangxi Zhuang autonomous region China (10124001A-49).

Conflicts of interest

None.

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2012

Authors and Affiliations

  • Yimin Zhu
    • 1
  • Xingyuan Xiao
    • 2
  • Lairong Dong
    • 1
  • Zhiming Liu
    • 3
  1. 1.Department of OncologySecond Hospital of JiaxingJiaxingChina
  2. 2.Department of General SurgeryFirst People’s Hospital of KunshanKunshanChina
  3. 3.Gastrointestine and Gland Surgery DivisionFirst Affiliated Hospital of Guangxi Medical UniversityNanningChina

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