Tumor Biology

, Volume 32, Issue 4, pp 631–639 | Cite as

Analysis of nuclear nestin localization in cell lines derived from neurogenic tumors

  • Olga KrupkovaJr
  • Tomas Loja
  • Martina Redova
  • Jakub Neradil
  • Karel Zitterbart
  • Jaroslav Sterba
  • Renata Veselska
Research Article


Nestin is a class VI intermediate filament protein expressed in the cytoplasm of stem and progenitor cells in the mammalian CNS during development. In adults, nestin is present only in a small subset of cells and tissues, including the subventricular zone of the adult mammalian brain, where neurogenesis occurs. Nestin expression has also been detected under such pathological conditions as ischemia, inflammation, and brain injury, as well as in various types of human solid tumors and their corresponding cell lines. Furthermore, nestin was recently found in the nuclei of glioblastoma, neuroblastoma, and angiosarcoma cells and it was proved to interact directly with the nuclear DNA in neuroblastoma cells. Here, we perform the first study of the intracellular distribution of nestin in cell lines derived from neurogenic tumors. Using immunodetection methods, we examined nestin expression in tumor-derived cell lines obtained from 11 patients with neuroblastoma, medulloblastoma, or glioblastoma multiforme. Besides its standard cytoplasmic localization, nestin was present in the nuclei of two neuroblastoma cell lines and one medulloblastoma cell line. Nestin was only present in the nuclei of cells with diffuse cytoplasmic staining for this protein, and the proportion of cells positive for nestin in nuclei, as well as the intensity of staining, varied. The presence of nestin in the nuclei was confirmed by both transmission electron microscopy and Western blotting. Our results indicate that the presence of nestin in the nuclei of tumor cells is not very rare, especially under in vitro conditions.


Nestin Intermediate filaments Cytoskeleton Glioblastoma multiforme Neuroblastoma Medulloblastoma 



We thank Dobromila Klemova and Johana Maresova for technical assistance. We also acknowledge Barbora Bujokova for confirming nestin expression in the SH-SY5Y cell line. This work was supported by Grant MUNI/A/0090/2009 of the Masaryk University as well as Grants IGA NR/9125-4 of the Ministry of Health of the Czech Republic and VZ MSM0021622415 of the Ministry of Education of the Czech Republic.

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2011

Authors and Affiliations

  • Olga KrupkovaJr
    • 1
  • Tomas Loja
    • 1
  • Martina Redova
    • 1
  • Jakub Neradil
    • 1
    • 2
  • Karel Zitterbart
    • 2
  • Jaroslav Sterba
    • 2
  • Renata Veselska
    • 1
    • 2
  1. 1.Laboratory of Tumor Biology and Genetics, Department of Experimental Biology, Faculty of ScienceMasaryk UniversityBrnoCzech Republic
  2. 2.Department of Pediatric OncologyMasaryk University and University Hospital BrnoBrnoCzech Republic

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