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Tumor Biology

, Volume 32, Issue 3, pp 561–568 | Cite as

Identification of differential expressed transcripts in cervical cancer of Mexican patients

  • Leticia Santos
  • Ma. Fabiola León-Galván
  • Erika Nahomy Marino-Marmolejo
  • Ana Paulina Barba de la Rosa
  • Antonio De León Rodríguez
  • Roberto González-Amaro
  • Ramón Gerardo Guevara-González
Research Article

Abstract

The aim of this study was to identify the gene expression profile in biopsies of patients with cervical intraepithelial neoplasia (CIN) 1, CIN 2, CIN 3, and microinvasive cancer by suppression subtractive hybridization and Southern blotting. After analyzing 1,800 cDNA clones, we found 198 upregulated genes, 166 downregulated, and no significant change of gene expression in 86 clones (p = 0.005). These results were validated by Northern blot analysis (p = 0.0001) in the identification of 28 overexpressed and 7 downregulated transcripts. We observed a set of genes related to the Notch signaling pathway that may be involved in the transformation of cervical cells and in the development to malignancy. The differentially expressed genes may provide useful information about the molecular mechanisms involved in human cervical carcinoma and as diagnostic markers.

Keywords

Cervical cancer SSH Gene expression Mexican women 

Notes

Acknowledgments

We thank Mrs. Sydney Robertson Jiménez for the English correction of the manuscript.

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2010

Authors and Affiliations

  • Leticia Santos
    • 1
    • 4
  • Ma. Fabiola León-Galván
    • 1
  • Erika Nahomy Marino-Marmolejo
    • 1
  • Ana Paulina Barba de la Rosa
    • 1
  • Antonio De León Rodríguez
    • 1
  • Roberto González-Amaro
    • 2
  • Ramón Gerardo Guevara-González
    • 3
  1. 1.División de Biologia Molecular, Instituto Potosino de Investigación Cientifica y Tecnologica A.C. (IPICYT)San Luis PotosiMexico
  2. 2.Departamento de Inmunología, Facultad de Medicina, UASLPSan Luis PotosíMexico
  3. 3.Departamento de Ingeniería Bioquímica, Instituto Tecnológico de CelayaCelayaMexico
  4. 4.San Luis PotosiMexico

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