Polymorphisms in the 5′- and 3′-untranslated region of the VEGF gene and sporadic breast cancer risk and clinicopathologic characteristics
- 152 Downloads
The wild and the variant alleles of the C936T and G634C vascular endothelial grow factor (VEGF) polymorphisms seem to be linked to higher angiogenic phenotype than the remaining alleles and may act on breast cancer (BC) origin. We investigated the influence of the VEGF C936T and G634C polymorphisms on the occurrence and clinicopathologic characteristics of sporadic breast cancer (SBC) in 235 patients and 235 controls. Peripheral blood samples of all individuals were analysed by the polymerase chain reaction for identification of genotypes and by enzyme-linked immunosorbent assay (ELISA) for quantification of serum VEGF levels. The variant 634CC genotype isolated (16.2% versus 10.7%, P = 0.01) and in combination with the wild 936CC genotype (10.6% versus 5.5%, P = 0.01) were more common in patients than in controls. The carriers of the respective genotypes were under a 2.20-fold and a 3.08-fold increased risks for the disease. Additionally, the frequency of the wild 936CC genotype was higher in patients with tumours of histological grade III compared to those with tumours of I+II histological grades (84.0% versus 64.7%, P = 0.004) and in patients with positive oestrogen receptor tumours compared to those with tumours lacking oestrogen receptor expression (84.7% versus 73.9%, P = 0.02). Similar serum values of VEGF were seen in patients and controls with the distinct genotypes of the VEGF. The data suggest that the VEGF wild 936CC and the variant 634CC genotypes constitute inherited determinants of SBC and SBC aggressiveness in Brazil, but are not significant predictors of circulating VEGF levels.
KeywordsBreast cancer Angiogenesis Polymorphism VEGF Risk
This work was supported by Fundação à Pesquisa do Estado de São Paulo (FAPESP).
Conflict of interest statement
We declare that we have no conflict of interest.
- 3.Hu Z, Fan C, Livasy C, et al. A compact VEGF signature associated with distant metastases and poor outcomes. BMC Med. 2009;16:7–9.Google Scholar
- 12.Langsenlehner U, Wolf G, Langsenlehner T, et al. Genetic polymorphisms in the vascular endothelial growth factor gene and breast cancer risk. The Austrian “tumor of breast tissue: incidence, genetics, and environmental risk factors” study. Breast Cancer Res Treat. 2008;109:297–304.CrossRefPubMedGoogle Scholar
- 17.Gu D, Wang M (2010) VEGF 936C>T polymorphism and breast cancer risk: evidence from 5,729 cases and 5,868 controls. Breast Cancer Res Treat 19. doi: 10.1007/s10549-010-0991-z
- 23.Brasil. Instituto Nacional de Câncer. Incidência de Câncer no Brasil. Estimativa 2010. Publishing Ministério da Saúde http://www.inca.gov.br/estimativa/2010
- 27.Yang DS, Park KH, Woo OH, Woo SU et al (2010) Association of a vascular endothelial growth factor gene 936C/T polymorphism with breast cancer risk: a meta-analysis. Breast Cancer Res Treat 21. doi: 10.1007/s10549-010-1070-1
- 29.Greene FL, Page DL, Fleming ID, et al. Breast. In: Balch CM, editor. AJCC Cancer staging manual. 6th ed. New York: Springer; 2002. p. 223–40.Google Scholar