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Tumor Biology

, Volume 32, Issue 1, pp 159–166 | Cite as

Expressions of hypoxia-inducible factor-1α and hexokinase-II in gastric adenocarcinoma: the impact on prognosis and correlation to clinicopathologic features

  • Miao-zhen Qiu
  • Bing Han
  • Hui-yan Luo
  • Zhi-wei Zhou
  • Zhi-qiang Wang
  • Feng-hua Wang
  • Yu-hong Li
  • Rui-hua Xu
Research Article

Abstract

The impact of hypoxia-inducible factor (HIF)-1α and hexokinase-II (HK-II) expression on prognosis of gastric adenocarcinoma patients has not been clearly established. We identified all patients in Cancer Center of Sun Yat-Sen University who were diagnosed as gastric adenocarcinoma and underwent radical gastrectomy between January 1999 and December 2001. We used immunohistochemistry to determine the expressions of HIF-1α protein and HK-II in the surgical sections. We identified 188 patients with gastric adenocarcinoma for the final analysis. The positive rate of HIF-1α and HK-II were 110/188 (54.6%) and 40/188 (21.3%), respectively. Both HIF-1α and HK-II were all positively correlated with tumor size, lower differentiation, and tumor stage. Univariate analysis showed that advanced tumor stages (P < 0.001), tumor size (P = 0.003), HIF-1α expression (P < 0.001), and HK-II expression (P < 0.001) were all significantly associated with shorter survival. The multivariate Cox analysis revealed that tumor stage (P < 0.001), HIF-1α expression (P < 0.001), and HK-II expression (P = 0.002) remained independent prognostic variables for survival. In addition, there was a positive correlation of HIF-1α protein expression and HK-II (P = 0.022). Both HIF-1α and HK-II were overexpressed in gastric adenocarcinoma. The multivariate Cox analysis revealed that both of them were independent factors on survival of gastric adenocarcinoma patients.

Keywords

HIF-1α HK-II Gastric adenocarcinoma Immunohistochemistry Survival 

Notes

Acknowledgments

These work was funded by National Natural Science Foundation of China grant 30672408, Guangzhou Bureau of Science and Technology grant 2006Z3-E0041 and Sun Yat-Sen University 985 Program Initiation Fund (China). We gratefully thank the staff members in the Department of Medical Oncology and GI Surgery Oncology at Sun Yat-Sen University Cancer Center for their suggestion and assistance.

Part of the results of this article was present in the 2008 ASCO annual meeting as a poster.

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2010

Authors and Affiliations

  • Miao-zhen Qiu
    • 1
    • 2
  • Bing Han
    • 1
    • 2
  • Hui-yan Luo
    • 1
    • 2
  • Zhi-wei Zhou
    • 1
    • 3
  • Zhi-qiang Wang
    • 1
    • 2
  • Feng-hua Wang
    • 1
    • 2
  • Yu-hong Li
    • 1
    • 2
  • Rui-hua Xu
    • 1
    • 2
  1. 1.State Key Laboratory of Oncology in South ChinaGuangzhouChina
  2. 2.Department of Medical OncologySun Yat-Sen University Cancer CenterGuangzhouChina
  3. 3.Department of GI SurgerySun Yat-Sen University Cancer CenterGuangzhouChina

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