Tumor Biology

, Volume 31, Issue 3, pp 217–224 | Cite as

RNAi-mediated ADAM9 gene silencing inhibits metastasis of adenoid cystic carcinoma cells

  • Qin Xu
  • Xiuming Liu
  • Yili Cai
  • Youcheng Yu
  • Wantao Chen
Research Article


A disintegrin and metalloproteinase 9 (ADAM9) is a type I transmembrane protein that has been associated with cancer development and metastases. Here, we show that ADAM9 is highly expressed in metastatic cancer tissues and in an adenoid cystic carcinoma cell line with a high metastatic potential. Using RNA interference for gene silencing, we show that ADAM9 is essential for in vitro cancer cell proliferation and invasion as well as in vivo cancer metastasis in an experimental murine model of lung metastases. These data indicate that ADAM9 is potentially an important new therapeutic target for the prevention of tumor metastases in adenoid cystic carcinoma.


ADAM9 Adenoid cystic carcinoma Gene silencing Metalloproteinase Metastasis 



A disintegrin and metalloproteinase


analysis of variance


dimethyl sulfoxide


epidermal growth factor receptor


fetal bovine serum


fibroblast growth factor receptor


glyceraldehyde-3-phosphate dehydrogenase


heparin-binding EGF-like growth factor


quantitative real-time polymerase chain reaction


specific pathogen free


transforming growth factor


tumor necrosis factor



This work was supported by the Chinese National Natural Science Foundation of China (Grant Number 30600715), Shanghai Leading Academic Discipline Project (Project Number S30206), and projects of the Shanghai Science and Technology Committee (Project Number 08JC 1404800, 08JC 1414400).


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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2010

Authors and Affiliations

  1. 1.Department of Oral and Maxillofacial Surgery, Ninth People’s HospitalShanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of StomatologyShanghaiChina
  2. 2.Department of Dentistry, Zhongshan HospitalFudan UniversityShanghaiChina

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