RNAi-mediated ADAM9 gene silencing inhibits metastasis of adenoid cystic carcinoma cells
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A disintegrin and metalloproteinase 9 (ADAM9) is a type I transmembrane protein that has been associated with cancer development and metastases. Here, we show that ADAM9 is highly expressed in metastatic cancer tissues and in an adenoid cystic carcinoma cell line with a high metastatic potential. Using RNA interference for gene silencing, we show that ADAM9 is essential for in vitro cancer cell proliferation and invasion as well as in vivo cancer metastasis in an experimental murine model of lung metastases. These data indicate that ADAM9 is potentially an important new therapeutic target for the prevention of tumor metastases in adenoid cystic carcinoma.
KeywordsADAM9 Adenoid cystic carcinoma Gene silencing Metalloproteinase Metastasis
A disintegrin and metalloproteinase
analysis of variance
epidermal growth factor receptor
fetal bovine serum
fibroblast growth factor receptor
heparin-binding EGF-like growth factor
quantitative real-time polymerase chain reaction
specific pathogen free
transforming growth factor
tumor necrosis factor
This work was supported by the Chinese National Natural Science Foundation of China (Grant Number 30600715), Shanghai Leading Academic Discipline Project (Project Number S30206), and projects of the Shanghai Science and Technology Committee (Project Number 08JC 1404800, 08JC 1414400).
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