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Iridin abrogates LPS-induced inflammation in L6 skeletal muscle cells by inhibiting NF-κB and MAPK signaling pathway

Abstract

Background

Iridin is a glycoside form of flavonoids isolated from Belamcanda chinensis, and Iris florentina has numerous biological properties, including antitumor, antiproliferative, anti-inflammatory and antioxidant properties. Inflammatory diseases are an essential health concern and have a growing incidence worldwide, so developing safe drugs remains an important objective.

Objective

In the present study, we evaluated iridin's anti-inflammatory effect on LPS-stimulated skeletal muscle cells (L6). The anti-inflammatory action of iridin elucidated through NF-κB (nuclear factor-kappa B) and MAPK (mitogen-activated protein kinases) pathway in LPS-stimulated L6 cells.

Results

Determination of cytotoxicity was examined by MTT assay. The expression level of cyclooxygenase‑2 (COX-2) and nitric oxide synthase (iNOS) was evaluated by western blot analysis. To elucidate the underlying mechanism, NF-κB and MAPKs proteins were investigated by immunoblot. Iridin could decrease the phosphorylation of NF-κB and MAPK proteins in LPS-induced L6 cells.

Conclusion

The results showed that iridin inhibited the phosphorylation of NF-κB and MAPK proteins and inflammatory cytokines COX-2 and iNOS in LPS-induced L6 cells. Based on findings in this work, iridin possesses anti-inflammatory action on L6 cells and could be considered for an attractive candidate for anti-inflammation.

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Acknowledgements

This study was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. NRF-2020R1F1A1069319) and the National Research Foundation of Korea funded by the Ministry of Science and ICT (Grant No. 2020R1A2B5B01001807).

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Contributions

PBB, SEH, JAK and GSK were involved in conceptualization; PBB was responsible for methodology; JAK and GSK performed validation; PBB, SEH, HHK and MYP carried out formal analysis; GSK undertook investigation; PBB and SEH wrote and prepared the original draft; AA contributed to writing, reviewing and editing; and JAK and GSK administered the project. All authors have read and agreed to the published version of the manuscript. All authors have read and agreed to the published version of the manuscript.

Corresponding authors

Correspondence to Gon Sup Kim or Jin-A. Kim.

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Conflict of interests

Pritam Bhagwan Bhosale, Sang Eun Ha, Hun Hwan Kim, Abuyaseer Abusaliya, Min Yeong Park, Gon Sup Kim, Jin-A Kim declares that they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Bhosale, P.B., Ha, S.E., Kim, H.H. et al. Iridin abrogates LPS-induced inflammation in L6 skeletal muscle cells by inhibiting NF-κB and MAPK signaling pathway. Mol. Cell. Toxicol. (2022). https://doi.org/10.1007/s13273-022-00277-3

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  • DOI: https://doi.org/10.1007/s13273-022-00277-3

Keywords

  • iNOS
  • COX-2
  • NF-κB
  • MAPK
  • And Iridin