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Transcriptional responses in Ecklonia cava to short-term exposure to polycyclic aromatic hydrocarbons

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Abstract

Backgrounds

Polycyclic aromatic hydrocarbons (PAHs) are the most persistent environmental toxicants, which can be found in every part of the environment. The PAHs are known to induce cancer, malformation, and mutation in various kinds of biological organisms, including humans. However, only limited toxic effects of PAHs in seaweeds have been accumulated so far.

Objectives

Differentially expressed genes (DEGs) against PAHs exposure were isolated using Ecava cDNA microarray and their biological functions were considered to anticipate the toxic effects of PAHs in Ecklonia cava.

Results

Gene ontology (GO) analysis of DEGs showed that PAHs exposure affected several processes, namely cellular protein metabolic, macromolecule biosynthetic, nucleic acid metabolic, carbohydrate derivative biosynthetic, glycoprotein metabolic processes, photosynthesis, and finally oxidoreductase activity in kelp species. Functional analysis of the most frequently observed DEGs in each of the 24-h and 48-h experimental groups revealed that PAHs appeared to induce stress and defense responses, as well as interfere with Wnt signaling pathways and biological molecule metabolism in E. cava.

Conclusion

This study demonstrated the discovery of DEGs in E. cava that may be impacted by environmental PAHs, as well as predicted how the transcript levels would affect physiology and metabolism. Such results will contribute to a better understanding of how seaweed reacts to pollution.

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Acknowledgements

This research was supported by a grant from the Ministry of Oceans and Fisheries of the Korean Government as “A sustainable research and development of Dokdo” project.

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Author notes

  1. Yejin Jo and Min Young Kim have contributed equally to this work.

Authors and Affiliations

  1. Ecological Risk Research Division, Korea Institute of Ocean Science and Technology (KIOST), Geoje, 53201, Republic of Korea

    Yejin Jo, Nayun Lee & Seungshic Yum

  2. GnC BIO, Banseok 36, Yuseong, Daejeon, 34068, Republic of Korea

    Min Young Kim & Hong-Seog Park

  3. Marine Biotechnology Research Center, Korea Institute of Ocean Science and Technology, Busan, 49111, Republic of Korea

    Seonock Woo

  4. KIOST School, University of Science and Technology, Geoje, 53201, Republic of Korea

    Seungshic Yum

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  1. Yejin Jo
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  2. Min Young Kim
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  4. Hong-Seog Park
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Contributions

YJ and NL performed experiments, analyzed the results, wrote the manuscript; MYK, H-SP carried out bioinformatic treatments, analyzed the results; SW and SY provided study conception, wrote the manuscript, and supervised the study.

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Correspondence to Seungshic Yum.

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Yejin Jo declares that she has no conflict of interest. Min Young Kim declares that she has no conflict of interest. Nayun Lee declares that she has no conflict of interest. Hong Seog Park declares that she has no conflict of interest. Seonock Woo declares that she has no conflict of interest. Seungshic Yum declares that he has no conflict of interest.

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Jo, Y., Kim, M.Y., Lee, N. et al. Transcriptional responses in Ecklonia cava to short-term exposure to polycyclic aromatic hydrocarbons. Mol. Cell. Toxicol. 18, 629–636 (2022). https://doi.org/10.1007/s13273-022-00262-w

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  • DOI: https://doi.org/10.1007/s13273-022-00262-w

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