Abstract
Background
PM2.5 is airborne particulate matter that is involved in air pollution and has become an important problem endangering human health. LncRNAs play important roles in malignant tumors. However, few studies have reported the mechanisms by which PM2.5 affects lung cancer.
Objective
To investigate the functional changes in PM2.5-exposed cells, we conducted tubule formation assays to determine angiogenesis and sequenced RNA to elucidate the key molecular effects of PM2.5 in Shenyang, China.
Results
RNA sequencing showed that the overall exposure values were very similar to the QPCR values. We found that 1379 lncRNA signatures and 162 mRNAs were differentially expressed between PM2.5-exposed and neutral saline-exposed cells. We further validated these changes via quantitative PCR in A549 human non-small-cell lung cancer (NSCLC) cell lines. PM2.5-exposed A549 cells presented carcinogenic transformation via lncRNA NONHSAT021963, which upregulates VEGF levels. The tubule formation assays showed that PM2.5-exposed cells were more angiogenic.
Conclusion
These results may help to clarify the proteins and signaling pathways affected by PM2.5 and lead to new diagnostic and therapeutic approaches in treating NSCLC.
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Abbreviations
- PM2.5:
-
Fine particulate matter
- ROS:
-
Reactive oxygen species
- HIF-1:
-
Hypoxia inducible factor-1
- VEGF-β:
-
Vascular endothelial growth factor-β
- PTGS2:
-
Prostaglandin endoperoxide synthase 2
- HSPA1B:
-
Heat shock 70 kDa protein 1B
- bFGF:
-
Basic fibroblast growth factor
- MMPs:
-
Matrix metalloproteinases
- IL24:
-
Interleukin24
- GAPDH:
-
Glyceraldehyde-3-phosphate dehydrogenase
- KEGG:
-
Kyoto Encyclopedia of Genes and Genomes
- NSCLC:
-
Non-small-cell lung cancer
- ECM:
-
Extracellular matrix
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Acknowledgement
The work was supported by the research funding from the Department of Science & Technology Liaoning (grant no. 2017225076 and 20180550862), the Shenyang Bureau of Science and Technology (no. 18-400-4-09), and Shenyang Medical College Fund Project (no. 20191030).
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BY and CX contributed conception and design of the study; BY carried out all experimental assays. HT and BY performed the statistical analysis; BY wrote the first draft of the manuscript; BY and HT wrote sections of the manuscript. All authors contributed to manuscript revision, read and approved the submitted version.
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Yang, B., Tian, H. & Xiao, C. lncRNA NONHSAT021963, which upregulates VEGF in A549 cells, mediates PM2.5 exposure-induced angiogenesis in Shenyang, China. Mol. Cell. Toxicol. (2020). https://doi.org/10.1007/s13273-020-00095-5
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DOI: https://doi.org/10.1007/s13273-020-00095-5