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The fate of hepatocyte cell line derived from a liver injury model with long-term in vitro passage

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Abstract

Backgrounds

Orthotopic Liver Transplantation (OLT) is the therapy of choice for the treatment of end-stage liver disease, but the severe shortage of organ donors, complex and expensive surgical procedure and increased mortality of prospective organ recipient limit the use of OLT. To overcome this problem the technique of hepatocyte transplantation has been considered as an alternative to OLT. Hepatocyte transplantation is less invasive, cost effective cryo-preservable and can be distributed from single donor to multiple recipients. In this study we have established and characterize the hepatocyte cell line possessing the morphological and functional characteristics of hepatocytes from chemically injured liver. Hence we hypothesized that the hepatocyte cell line derived from liver injury can be used for hepatocyte transplantation.

Methods

To induce the priming of hepatocyte, mice were fed with 3,5-diethoxycarbonyl-1,4-dihydro-collidine (DDC) diet for 3 weeks and hepatocytes were obtained by two step collagenase perfusion method, hepatocytes hence obtained was expended by >300 passages and tested for various hepatocyte specific functions.

Results

The cell line derived from liver injury model retains morphological and functional characteristics of hepatocytes in long-term in vitro culture. These cells transplanted to mice showed significant survival rate.

Conclusion

The Hepatocyte cell line derived from liver injury model were used for hepatocyte transplantation and showed the significantly higher survival rate.

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Authors and Affiliations

  1. Laboratory of Liver Regeneration, Biomedical Research Institute, Chonbuk National University Medical School, 634-18 Geumam-dong, Duckjin-gu, Jeonju, 54907, Republic of Korea

    Lokendra Chand, Nirajan Shrestha, Dhruba Acharya & Yeon Jun Jeong

  2. Department of Biochemistry, Kathmandu University School of Medical Sciences, Dhulikhel, Kavre, Nepal

    Prabodh Risal

  3. Department of Microbiology, Kathmandu University School of Medical Sciences, Dhulikhel, Kavre, Nepal

    Dhruba Acharya

  4. Department of Pediatrics, Elmhurst Hospital, Illinois, USA

    Yae Sul Jeong

  5. Department of Surgery, Chonbuk National University Hospital, 634-18 Geumam-dong, Duckjin-gu, Jeonju, 54907, Republic of Korea

    Chan Young Kim & Yeon Jun Jeong

  6. Department of Pediatrics, Icahn School of Medicine at Mount Sinai, New York, USA

    Yae Sul Jeong

Authors
  1. Lokendra Chand
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  2. Prabodh Risal
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  3. Nirajan Shrestha
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  4. Dhruba Acharya
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  5. Yae Sul Jeong
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  6. Chan Young Kim
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  7. Yeon Jun Jeong
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Correspondence to Yeon Jun Jeong.

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Chand, L., Risal, P., Shrestha, N. et al. The fate of hepatocyte cell line derived from a liver injury model with long-term in vitro passage. Mol. Cell. Toxicol. 14, 263–272 (2018). https://doi.org/10.1007/s13273-018-0029-x

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  • DOI: https://doi.org/10.1007/s13273-018-0029-x

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