Igongsan reduces testosterone-induced benign prostate hyperplasia by regulating 5α-reductase in rats
- 42 Downloads
Igongsan (IGS) is a traditional Korean herbal medication composed of five different herbs; Citri Unshius Pericarpium, Poria Sclerotium, Glycyrrhizae Radix et Rhizoma, Atractylodis Rhizoma Alba, and Ginseng Radix. In this study, we evaluated the effect of IGS on benign prostatic hyperplasia (BPH), a disease resulting from a noncancerous size increase of the prostate which is common in aging men.
We induced BPH by a 4-week daily injection of testosterone propionate and investigated the effects IGS on BPH. After pre-treatment, the rats were divided into four groups and treated by each drugs for 4 weeks. Histological alteration was observed by hematoxylin and eosin (H&E) staining. Type-2 5α-reductase (5AR-2), androgen receptor (AR), estrogen receptor-α (ERα) and prostate specific antigen (PSA) were confirmed by western blot analysis and immunohistochemistry staining.
IGS reduced the enlarged prostate and prostatic index, while the epithelium thickness and enlarged lumen area returned to their normal state in BPH-induced rats. In particular, 5AR-2, which is a major target for BPH medication, was inhibited by IGS. IGS also regulated the factors including AR and ERα to interact with 5AR-2. Consequently, PSA, a major diagnostic marker for BPH, was suppressed by IGS treatment.
Based on our findings, this study shows that IG S can alleviate BPH by regulating 5AR, suggesting its potential as a new, effective medication for BPH treatment.
KeywordsBenign prostate hyperplasia Igongsan Type-2 5α-reductase Androgen receptor Estrogen receptor α Prostate specific antigen
Unable to display preview. Download preview PDF.
- 22.Rho, Y., Ahn, K., Chang, S., Jung, J. & Lee, K. Preventive Effect of Crude Drug Preparation (E-kong-san) on Cisplatin induced Nephrotoxicity. Korean J Pharmacogn 29, 258–264 (1998)Google Scholar
- 23.Lee, K. et al. Cytoprotective and Antioxidative Effects of Crude Drug Preparation (E-kong-san). Korean J Pharmacogn 30, 255–260 (1999)Google Scholar
- 24.Gene, M. E. et al. Anti-metastatic and Anti-angiogenic Activities of Ekong-san and Its Metabolites by Human Intestinal Bacteria. J Physiol & Pathol Korean Med 18, 1686–1693 (2004)Google Scholar
- 25.Park, S., Kim, K., Jung, H., Choi, W. & Yoon, S. The Study on the Process and Quality Control of Rhus Verniciflua Stokes Extract (Nexia). J of Kor Orilental Oncol 11, 31–39 (2006)Google Scholar
- 26.Ozer, K., Horsanali, M. O., Gorgel, S. N., Horsanali, B. O. & Ozbek, E. Association between Benign Prostatic Hyperplasia and Neutrophil-Lymphocyte Ratio, an Indicator of Inflammation and Metabolic Syndrome. Urol Int doi:10.1159/000448289 (2016).Google Scholar
- 31.Kim, B. H., Sohn, J. C., Park, C. H. & Kim, C. I. The usefulness of intravesical prostatic protrusion and bladder wall thickness measurement using transabdominal ultrasound in patients with benign prostatic hyperplasia. Korean J Urol 46, 1180–1185 (2005).Google Scholar
- 38.Gandaglia, G. et al. The role of prostatic inflammation in the development and progression of benign and malignant diseases. Curr Opin Urol doi:10.1159/000448289 (2016).Google Scholar
- 40.Hong, E. et al. Eucommia ulmoides extract stimulates glucose uptake through PI 3-kinase mediated pathway in L6 rat skeletal muscle cells. Mol Cell Toxicol 4, 224–229 (2008).Google Scholar