Effects of waterborne copper on toxicity stress and apoptosis responses in red seabream, Pagrus major
- 36 Downloads
There has been an increasing trend of copper (Cu)-based aquaculture industry in the world, and it is necessary to evaluate the effect of copper ion exposure on water pollution. This study was aim to determine the critical concentration of toxic copper in adult red sea bream.
Therefore, we investigated the effects of Cu toxicity on physiological stress and apoptosis exposed to various concentrations (10, 20, 30, and 40 μg/L). We measured physiological stress-related (corticotrophin- releasing hormone, adrenocorticotropic hormone, cortisol, and glucose), other toxic stress-related (metallothionein and Na+/K+-ATPase), and apoptosis-related (caspase-3 and hydrogen peroxide) parameters. In addition, we confirmed apoptosis.
Physiological parameters were significantly increased from Cu concentration of 30 μg/L or more. However, no significant differences were observed after exposures at 10 and 20 μg/L. In addition, apoptotic cells were detected after exposure to 30 μg/L.
The results of this study indicate that high concentrations induce stress and apoptosis compared to normal conditions.
KeywordsApoptosis Copper HPI axis Physiological stress Toxicity stress
Unable to display preview. Download preview PDF.
- 4.Incheon metropolitan city hall. http://www.incheon. go.kr/program/fileDownload.do?file No=581105.pdf (2016).Google Scholar
- 5.Drach, A. Utilization of Copper Alloys for Marine Applications (Doctoral dissertation, University of New Hampshire, 2013).Google Scholar
- 6.Tellis, M. S., Alsop, D. & Wood, C. M. Effects of copper on the acute cortisol response and associated physiology in rainbow trout. Comp Biochem Physiol C 155, 281–289 (2012).Google Scholar
- 14.Sampaio, F. G. et al. Antioxidant defenses and biochemical changes in the neotropical fish pacu, Piaractus mesopotamicus: Responses to single and combined copper and hypercarbia exposure. Comp Biochem Physiol C 156, 178–186 (2012).Google Scholar
- 19.Meena, B., Suresh, A., Sumit, R. & Mani, R. Induction of metallothionein with cadmium chloride in a economically important freshwater fish-grass carp, Ctenopharyngodon idella (valenciennes, 1844). Asian J Pharm Clin Res 8, 276–281 (2015).Google Scholar
- 20.Wu, S. M. Cortisol and Copper Induce Metallothionein Expression in Three Tissues of Tilapia (Oreochromis mossambicus) in Organ Culture. Zool stud 45, 363–370 (2006).Google Scholar
- 32.Donaldson, E. M. & Dye, H. M. Corticosteroid concentrations in sockeye salmon (Oncorhynchus nerka) exposed to low concentrations of copper. Can J Fish Aquat Sci 32, 533–539 (1975).Google Scholar
- 33.Hedayati, A. & Ghaffari, Z. Evaluation of the Effects of Exposure to Copper Sulfate on some Eco-physiological Parameters in Silver Carp (Hypophthalmichthys molitrix). Iranian Journal of Toxicology 7, 887–893 (2013).Google Scholar
- 37.Trivedi, M. H., Sangai, N. P. & Renuka, A. Assessment of toxicity of copper sulphate pentahydrate on oxidative stress indicators on liver of gold fish (Carassius auratus). Bull Environ Pharmacol Life Sci 1, 52–57 (2012).Google Scholar
- 38.Umasuthan, N. et al. A manganese superoxide dismutase (MnSOD) from Ruditapes philippinarum: Comparative structural-and expressional-analysis with copper/zinc superoxide dismutase (Cu/ZnSOD) and biochemical analysis of its antioxidant activities. Fish Shellfish Immunol 33, 753–765 (2012).CrossRefPubMedGoogle Scholar