Molecular & Cellular Toxicology

, Volume 14, Issue 2, pp 143–153 | Cite as

Low-dose diethylhexyl phthalate exposure does not impair the expressive patterns of epigenetics-related genes and DNA methylation of breast cancer-related genes in mouse mammary glands

  • Shun-Feng Cheng
  • Ling Li
  • Bo Li
  • Jing-Cai Liu
  • Fang-Nong Lai
  • Yong Zhao
  • Xi-Feng Zhang
  • Wei Shen
  • Lan Li
Original Paper



Di-(2-ethylhexyl) phthalate (DEHP), as an endocrine-disrupting chemical (EDC), is widely used in plasticizer and other productions. Ubiquitous human exposure to DEHP has been proposed to be a potential risk to public health. Developmental exposure to DEHP could alter epigenetic programming and result in adult-onset disease.


In this study, we investigated whether DEHP exposure to pregnant mice affected epigenetic changes as a result of increase in breast cancer incidence.


Our results showed that the expression of total 143 epigenetics-related genes in mammary gland cells, have no significantly altered after short time and low-dose treated with DEHP from 0.5 days post-coitum (dpc) to 3.5 dpc of pregnant mice. DNA methylation status of some neoplastic development genes, such as EGFr, Esr1, Pgr, Fos and Rassf5 also had no obvious change.


These finding showed no impact of DEHP on the expressive patterns of epigenetics-related genes and DNA methylation of breast cancer-related genes in pregnant mouse mammary gland cells.


DEHP Mammary gland cells Epigenetics DNA methylation 


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Supplementary material

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Low-dose diethylhexyl phthalate exposure does not impair the expressive patterns of epigenetics-related genes and DNA methylation of breast cancer-related genes in mouse mammary glands


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Copyright information

© The Korean Society of Toxicogenomics and Toxicoproteomics and Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Shun-Feng Cheng
    • 1
  • Ling Li
    • 2
  • Bo Li
    • 3
  • Jing-Cai Liu
    • 1
  • Fang-Nong Lai
    • 1
  • Yong Zhao
    • 1
  • Xi-Feng Zhang
    • 4
  • Wei Shen
    • 1
  • Lan Li
    • 1
  1. 1.Institute of Reproductive Sciences, College of Animal Science and TechnologyQingdao Agricultural UniversityQingdaoChina
  2. 2.Tengzhou People’s HospitalTengzhouChina
  3. 3.Chengguo Station of Animal Husbandry and VeterinaryLaizhouChina
  4. 4.College of Biological and Pharmaceutical EngineeringWuhan Polytechnic UniversityWuhanChina

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