Abstract
Osteosarcoma has been reported as a severe and worldwide clinical problem, research focusing on osteosarcoma for overall analysis and understanding of the mechanism will be of great importance for theoretical basis and treatment of osteosarcoma. In the present study, we sought to explore the correlation of miR-200-3p with osteosarcoma proliferation, apoptosis and the related signaling pathway. MTT, flow cytometry, qRT-PCR and western blot were separately used to test the influence of miR-200-3p on cells proliferation, cells apopsotis and the FOXO expression. The results shown that miR-200-3p inhibits cell viability, and promotes apoptosis in Saos-2 and U2OS. The wertern blot results proved the overexpression of miR-200-3p can activate the FOXO signaling pathway by upregulating FOXO1 expression.
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Jiang, J., Jiang, J., Yang, Y. & Cao, J. Identification of microRNAs potentially involved in male sterility of Brassica campestris ssp. chinensis using microRNA array and quantitative RT-PCR assays. Cell Mol Biol Lett 18: 416–432 (2013).
Khuu, C., Utheim, T. P. & Sehic, A. The Three Paralogous MicroRNA Clusters in Development and Disease, miR-17-92, miR-106a-363, and miR-106b-25. Scientifica 2016: 1–10 (2016).
Schaefer, J. S. MicroRNAs: how many in inflammatory bowel disease? Curr Opin Gastroenterol 32: 258–266 (2016).
Schaefer, J. S. et al. MicroRNA signatures differentiate Crohn’s disease from ulcerative colitis. BMC Immunol 16: 1–13 (2015).
Chen, W. X., Ren, L. H. & Shi, R. H. Implication of miRNAs for inflammatory bowel disease treatment: Systematic review. World J Gastrointest Pathophysiol 5: 63–70 (2014).
Xu, D. et al. MicroRNA-383 expression regulates proliferation, migration, invasion, and apoptosis in human glioma cells. Tumor Biol 36: 1–11 (2015).
Wang, K. MiR-92b controls glioma proliferation and invasion through regulating Wnt/beta-catenin signaling via Nemo-like kinase. Neuro-Oncology 15: 970 (2013).
Peng, G., Liao, Y. & Shen, C. miRNA-429 Inhibits Astrocytoma Proliferation and Invasion by Targeting BMI1. (2016).
Li, X. et al. MiR-16-1 Plays a Role in Reducing Migration and Invasion of Glioma Cells. Anat Rec 296: 427–432 (2013).
Kumar, R. M. R., Boro, A. & Fuchs, B. Involvement and Clinical Aspects of MicroRNA in Osteosarcoma. Int J Mol Sci 17:E877 (2016).
Sampson, V. B., Yoo, S., Kumar, A., Vetter, N. S. & Kolb, E. A. MicroRNAs and Potential Targets in Osteosarcoma: Review. Front Pediatr 3: 69 (2015).
Tian, Y., Zhang, Y. Z. & Chen, W. MicroRNA-199a-3p and microRNA-34a regulate apoptosis in human osteosarcoma cells. Bioscience Rep 34: 479–485 (2014).
Novello, C. et al. p53-Dependent Activation of microRNA-34a in Response to Etoposide-Induced DNA Damage in Osteosarcoma Cell Lines Not Impaired by Dominant Negative p53 Expression. Plos One 9: e114757–e114757 (2014).
Sun, H. et al. Expression analysis of microRNAs related to the skin ulceration syndrome of sea cucumber Apostichopus japonicus. Fish Shellfish Immun 49: 205–212 (2015).
Xiao, Y. et al. p38/p53/miR-200a-3p feedback loop promotes oxidative stress-mediated liver cell death. Cell Cycle 14: 1548–1558 (2015).
Pautke, C. et al. Characterization of osteosarcoma cell lines MG-63, Saos-2 and U-2 OS in comparison to human osteoblasts. Anticancer Res 24: 3743–3748 (2004).
Dumnoensun, P. et al. Overexpression of KH-type splicing regulatory protein regulates proliferation, migration, and implantation ability of osteosarcoma. Int J Oncol 49: 903–912 (2016).
Avnet, S. et al. Altered pH gradient at the plasma membrane of osteosarcoma cells is a key mechanism of drug resistance. Oncotarget 7: 63408–63423 (2016).
Robl, B., Botter, S. M., Pellegrini, G., Neklyudova, O. & Fuchs, B. Evaluation of intraarterial and intravenous cisplatin chemotherapy in the treatment of metastatic osteosarcoma using an orthotopic xenograft mouse model. J Exp Clin Cancer Res 35: 1–14 (2016).
Li, C.-j. et al. Advances in Bone-targeted Drug Delivery Systems for Neoadjuvant Chemotherapy for Osteosarcoma. Orthop Surg 8: 105–110 (2016).
Hattinger, C. M. et al. Advances in emerging drugs for osteosarcoma. Expert Opin Emerg Drugs 20: 1–20 (2015).
Vos, H. I., Coenen, M. J., Guchelaar, H. J. & Te Loo, D. M. The role of pharmacogenetics in the treatment of osteosarcoma. Drug Discov Today 21: 1775–1786 (2016).
Wu, X. et al. MicroRNA-34a Inhibits Human Osteosarcoma Proliferation by Downregulating Ether à go-go 1 Expression. Int J Med Sci 10: 676–682 (2013).
Wang, Z., Rongzhen, H. E., Xia, H., Wei, Y. U. & Song, W. U. MicroRNA-101 has a suppressive role in osteosarcoma cells through the targeting of c-FOS. Exp Ther Med 11: 1293–1299 (2016).
Zhi, X. et al. MicroRNA-494 inhibits proliferation and metastasis of osteosarcoma through repressing insulin receptor substrate-1. Am J Transl Res 8: 3439–3447 (2016).
Chen, X. et al. Identification of Dietetically Absorbed Rapeseed (Brassica campestris L.) Bee Pollen MicroRNAs in Serum of Mice. Biomed Res Int 2016: 5413849 (2016).
Liang, L. et al. Cryptotanshinone inhibits human glioma cell proliferation by suppressing STAT3 signaling. Mol Cell Biochem 381: 273–282 (2013).
Nam, K. S., Oh, S., Lee, K. M., Yoo, S. A. & Shin, I. CD44 regulates cell proliferation, migration, and invasion via modulation of c-Src transcription in human breast cancer cells. Cell Signal 27: 1882–1894 (2015).
Du, M. D., He, K. Y., Qin, G., Chen, J. & Li, J. Y. Adriamycin resistance-associated prohibitin gene inhibits proliferation of human osteosarcoma MG63 cells by interacting with oncogenes and tumor suppressor genes. Oncol Lett 12: 1994–2000 (2016).
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Li, Y., Wu, Y. MiR-200-3p inhibits tumor cell proliferation and induces apoptosis by upregulation of FOXO1 in osteosarcoma cells. Mol. Cell. Toxicol. 14, 73–78 (2018). https://doi.org/10.1007/s13273-018-0009-1
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DOI: https://doi.org/10.1007/s13273-018-0009-1