Skip to main content
SpringerLink
Log in

The effect of purple rice (Oryza sativa L. indica) extract on the inflammatory response in a colon cancer cell line and dextran sulfate-induced tumor promotion in the rat colon

  • Original Paper
  • Published:
Molecular & Cellular Toxicology Aims and scope Submit manuscript

Abstract

This study investigated the effects of purple rice extract (PRE ) on inflammation-related colon carcinogenesis. Administration of PRE 1 g/kg bodyweight reduced both the number (39.05%) and size of aberrant crypt foci (ACF) (14.28%) in the colons of rats that received dimethylhydrazine (DMH) initiation with promotion by dextran sulfate sodium (DSS) (P<0.05) with the reduction of TNF-α expression in colonic epithelial cells. On the other hand, PRE could suppress the expression of both TNF-α and IL-6 in RAW 264.7 cells activated by lipopolysaccharide (LPS) at both the mRNA and protein levels. Moreover it could reduce the expression of TNF-α and IL-1β mRNA in inflamed HT-29. These findings suggested that PRE is able to modulate the inflammatory process in both activated macrophages and colon cancer cells. Therefore, PRE suppressed macrophage activation and colon cancer cell line response to inflammation and modulated of inflammation induced ACF progression in the rat colon.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Attasara, P. & Buasom, R. in Hospital-based cancer registry annual report 2012 (National Cancer Institute, Bangkok, 2014).

    Google Scholar 

  2. Klampfer, L. Cytokines, Inflammation and Colon Cancer. Curr Cancer Drug Targets 11:451–464 (2011).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Askling, J. et al. Family history as a risk factor for colorectal cancer in inflammatory bowel disease. Gastroenterology 120:1356–1362 (2001).

    Article  CAS  PubMed  Google Scholar 

  4. Colotta, F. et al. Cancer-related inflammation, the seventh hallmark of cancer: links to genetic instability. Carcinogenesis 30:1073–1081 (2009).

    Article  CAS  PubMed  Google Scholar 

  5. Burkholder, B. et al. Tumor-induced perturbations of cytokines and immune cell networks. BBA-Rev Cancer 1845:182–201 (2014).

    CAS  Google Scholar 

  6. Fearon, E. R. & Vogelstein, B. A genetic model for colorectal tumorigenesis. Cell 61:759–767 (1990).

    Article  CAS  PubMed  Google Scholar 

  7. Morin, P. J. et al. Activation of beta-catenin-Tcf signaling in colon cancer by mutations in beta-catenin or APC. Science 275:1787–1790 (1997).

    Article  CAS  PubMed  Google Scholar 

  8. Pan, M.-H., Lai, C.-S. & Ho, C.-T. Anti-inflammatory activity of natural dietary flavonoids. Food Funct 1:15–31 (2010).

    Article  CAS  PubMed  Google Scholar 

  9. Terzic, J., Grivennikov, S., Karin, E. & Karin, M. Inflammation and colon cancer. Gastroenterology 138: 2101–2114 (2010).

    Article  CAS  PubMed  Google Scholar 

  10. Mantovani, A., Allavena, P., Sica, A. & Balkwill, F. Cancer-related inflammation. Nature 454:436–444 (2008).

    Article  CAS  PubMed  Google Scholar 

  11. Raffoul, J. J., Kucuk, O., Sarkar, F. H. & Hillman, G. G. Dietary Agents in Cancer Chemoprevention and Treatment. J Oncol 2012:2 (2012).

    Google Scholar 

  12. Boonsit, P., Pongpiachan, P., Julsrigival, S. & Karladee, D. Gamma Oryzanol Content in Glutinous Purple Rice Landrace Varieties. Chiang Mai J Sci 9:151–157 (2010).

    Google Scholar 

  13. Punyatong, M., Pongpiachan, P., Karladee, D. & Mankhetkorn, S. Cytotoxicity of crude proanthocyanidin extract from purple glutinous rice bran (Oryza sativa L.) (Kum Doi Saket) compared with cyanidin 3-glucoside on X63 myeloma cancer cell lines. Nat Sci 42:676–681 (2008).

    Google Scholar 

  14. Apichai, S. et al. Evaluation of Antidiabetic Efficacy of Thai Purple Sticky Rice in High-Fat Diet plus Streptozotocin (STZ) Induced Type 2 Diabetic Rats. The 3 rd International Conference on International Relations and Development (ICIRD 2013), Chiang Mai, Thailand. August 8-10, 2013.

  15. Summart, R. & Chewonarin, T. Purple rice extract supplemented diet reduces DMH-induced aberrant crypt foci in the rat colon by inhibition of bacterial beta-glucuronidase. Asian Pac J Cancer Prev 15:749–755 (2014).

    Article  PubMed  Google Scholar 

  16. Wongjaikam, S., Summart, R. & Chewonarin, T. Apoptosis Induction in Colon Cancer Cell Lines and Alteration of Aberrant Crypt Foci in Rat Colon by Purple Rice (Oryza sativa L. var. glutinosa) Extracts. Nutr Cancer 66:690–699 (2014).

    Article  PubMed  Google Scholar 

  17. Tu, J. et al. TanshinoneIIA ameliorates inflammatory microenvironment of colon cancer cells via repression of microRNA -155. Int Immunopharmacol 14:353–361 (2012).

    Article  CAS  PubMed  Google Scholar 

  18. Punvittayagul, C., Sringarm, K., Chaiyasut, C. & Wongpoomchai, R. Mutagenicity and antimutagenicity of hydrophilic and lipophilic extracts of Thai northern purple rice. Asian Pac J Cancer Prev 15:9517–9522 (2014).

    Article  PubMed  Google Scholar 

  19. Banjerdpongchai, R., Wudtiwai, B. & Sringarm, K. Cytotoxic and apoptotic-inducing effects of purple rice extracts and chemotherapeutic drugs on human cancer cell lines. Asian Pac J Cancer Prev 14:6541–6548 (2013).

    Article  Google Scholar 

  20. Saenjum, C. et al. Antioxidant and anti-inflammatory activities of gamma-oryzanol rich extracts from Thai purple rice bran. J Med Plants Res 6:1070–1077 (2012).

    CAS  Google Scholar 

  21. Xu, Z., Hua, N. & Godber, J. S. Antioxidant Activity of Tocopherols, Tocotrienols, and γ-Oryzanol Components from Rice Bran against Cholesterol Oxidation Accelerated by 2,2'-Azobis(2-methylpropionamidine) Dihydrochloride. J Agr Food Chem 49:2077–2081 (2001).

    Article  CAS  Google Scholar 

  22. Feregrino-Perez, A. A. et al. A non-digestible fraction of the common bean (Phaseolus vulgaris L.) induces cell cycle arrest and apoptosis during early carcinogenesis. Plant foods Hum Nutr 69:248–254 (2014).

    Article  CAS  PubMed  Google Scholar 

  23. Brown, W. A. et al. Non-steroidal anti-inflammatory drugs with different cyclooxygenase inhibitory profiles that prevent aberrant crypt foci formation but vary in acute gastrotoxicity in a rat model. J gastroenterol hepatology 15:1386–1392 (2000).

    Article  CAS  Google Scholar 

  24. Puangpraphant, S. et al. Yerba mate tea and mate saponins prevented azoxymethane-induced inflammation of rat colon through suppression of NF-kappaB p65ser(311) signaling via IkappaB-alpha and GSK-3beta reduced phosphorylation. BioFactors 39:430–440 (2013).

    Article  CAS  PubMed  Google Scholar 

  25. Kohno, H. et al. Ursodeoxycholic Acid versus Sulfasalazine in Colitis-Related Colon Carcinogenesis in Mice. Clin Cancer Res 13:2519–2525 (2007).

    Article  CAS  PubMed  Google Scholar 

  26. Saxena, A. et al. Adiponectin deficiency: Role in chronic inflammation induced colon cancer. BBA-Mol Basis Dis 1822:527–536 (2012).

    Article  CAS  Google Scholar 

  27. Lewis, C. E. & Pollard, J. W. Distinct role of macrophages in different tumor microenvironments. Cancer Res 66:605–612 (2006).

    Article  CAS  PubMed  Google Scholar 

  28. Kahkonen, M. P. et al. Antioxidant activity of plant extracts containing phenolic compounds. J Agric Food Chem 47:3954–3962 (1999).

    Article  CAS  PubMed  Google Scholar 

  29. Kumar, S. et al. Antioxidant and free radical scavenging potential of Citrullus colocynthis (L.) Schrad. methanolic fruit extract. Acta Pharm 58:215–220 (2008).

    CAS  PubMed  Google Scholar 

  30. Bird, R. P. Role of aberrant crypt foci in understanding the pathogenesis of colon cancer. Cancer Lett 93:55–71 (1995).

    Article  CAS  PubMed  Google Scholar 

  31. Bird, R. P. Observation and quantification of aberrant crypts in the murine colon treated with a colon carcinogen: Preliminary findings. Cancer Lett 37:147–151 (1987).

    Article  CAS  PubMed  Google Scholar 

  32. Paul, S. et al. Anti-inflammatory action of pterostilbene is mediated through the p38 mitogen-activated protein kinase pathway in colon cancer cells. Cancer Prev Res 2:650–657 (2009).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

    Warunyoo Phannasorn, Chakkrit Khanaree, Ariyaphong Wongnoppavich & Teera Chewonarin

Authors
  1. Warunyoo Phannasorn
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chakkrit Khanaree
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ariyaphong Wongnoppavich
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Teera Chewonarin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Teera Chewonarin.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Phannasorn, W., Khanaree, C., Wongnoppavich, A. et al. The effect of purple rice (Oryza sativa L. indica) extract on the inflammatory response in a colon cancer cell line and dextran sulfate-induced tumor promotion in the rat colon. Mol. Cell. Toxicol. 13, 433–442 (2017). https://doi.org/10.1007/s13273-017-0047-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13273-017-0047-0

Keywords

Search

Navigation