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Substance-P protects intestinal epithelium against dextran sulfate sodium-induced toxicity in vitro

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Abstract

Dextran sulfate sodium (DSS) has been used to destroy intestinal epithelium structure and impair barrier function to mimic clinic intestine damages, which causes the intestinal inflammation and furthermore, tissue destruction. In this study, we have attempted to damage intestinal epithelium by treating DSS to Caco-2, human intestinal epithelial cell line, and elucidated the recovery effect of SP in vitro. DSS altered cellular morphology with the loss of TJ and reduced cell viability. Notably, DSS arrested cell cycle to decrease proliferating cell pool. However, SP treatment could preserve cellular morphology and TJ expression level similar to the normal even under DSS-induced toxicity. SP was also able to enhance cell survival, accompanied by the accelerated cell proliferation. Collectively, our study revealed SP can block the intestine damage due to toxicity of DSS. Our results provide the possibility of SP as a potential therapeutic for intestinal damage.

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Authors and Affiliations

  1. Department of Medicine, Graduate School, Kyung Hee University, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Republic of Korea

    Dae Yeon Hwang

  2. Graduate School of Biotechnology & Department of Genetic Engineering, College of Life Science, Kyung Hee University, Seochundong, Kiheung-gu, Yong In, 17104, Republic of Korea

    Ju Hyeong Park & Youngsook Son

  3. Department of Clinical Pharmacology, School of Medicine, Kyung Hee University, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Republic of Korea

    Sung-Vin Yim

  4. College of Medicine/East-West Medical Research Institute, Kyung Hee University, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Republic of Korea

    Hyun Sook Hong

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  1. Dae Yeon Hwang
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Correspondence to Hyun Sook Hong.

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Hwang, D.Y., Park, J.H., Yim, SV. et al. Substance-P protects intestinal epithelium against dextran sulfate sodium-induced toxicity in vitro . Mol. Cell. Toxicol. 12, 391–398 (2016). https://doi.org/10.1007/s13273-016-0043-9

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  • DOI: https://doi.org/10.1007/s13273-016-0043-9

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