Abstract
Although extract of Dermatophagoides farinae (DF) alone had no effect on neutrophil apoptosis, it inhibited neutrophil apoptosis in neutrophils cocultured with lymphocytes in both normal and allergic subjects. DF showed a stronger inhibition on the apoptosis of allergic neutrophils cocultured with allergic lymphocytes than that of normal neutrophils cocultured with normal lymphocytes. DF induced the secretion of IL-6, IL-8, MCP-1, and GM-CSF of the normal and allergic lymphocytes. The cytokine secretion due to DF in allergic lymphocytes is higher than in normal lymphocytes. The cytokine secretion of normal and allergic lymphocytes induced by DF was suppressed by PAR2i, a PAR2 inhibitor, LY294002, a PI3K inhibitor, AKTi, an Akt inhibitor, PD98059, an ER K inhibitor, and BAY-11-7085, an NF-κB inhibitor. Phosphorylation of ERK induced by DF was suppressed by PAR2i, LY294002 and AKTi, and NF-κB activity due to DF was inhibited by PAR2i, LY294002, AKTi, and PD98059.
Similar content being viewed by others
References
Kim, I. S., Kim, M. J., Kim, D. H., Choi, E. & Lee, J. S. Different anti-apoptotic effects of normal and asthmatic serum on normal eosinophil apoptosis depending on house dust mite-specific IgE. Mol Biol Rep 40:5875–5881 (2013).
Kim, I. S., Kim, D. H., Yun, C. Y. & Lee, J. S. A (S)-(+)-decursin derivative, (S)-(+)-3-(3,4-dihydroxyphenyl)-acrylic acid 2,2-dimethyl-8-oxo-3,4-dihydro-2H,8H-pyrano[3,2-g]-chromen-3-yl-ester, attenuates the development of atopic dermatitis-like lesions in NC/Nga mice. Mol Biol Rep 40:2541–2548 (2013).
Thomas, W. R., Hales, B. J. & Smith, W. A. House dust mite allergens in asthma and allergy. Trends Mol Med 16:321–328 (2010).
Sporik, R., Chapman, M. D. & Platts-Mills, T. A. House dust mite exposure as a cause of asthma. Clin Exp Allergy 22:897–906 (1992).
Thomas, W. R., Smith, W. A., Hales, B. J., Mills, K. L. & O’Brien, R. M. Characterization and immunobiology of house dust mite allergens. Int Arch Allergy Immunol 129:1–18 (2002).
Jacquet, A. The role of innate immunity activation in house dust mite allergy. Trends Mol 17:604–611 (2011).
Tsai, J. J. et al. Mite allergen Der-p2 triggers human B lymphocyte activation and Toll-like receptor-4 induction. PLoS One 6:e23249 (2011).
Liu, C. F., Drocourt, D., Puzo, G., Wang, J. Y. & Riviere, M. Innate immune response of alveolar macrophage to house dust mite allergen is mediated through TLR2/-4 co-activation. PLoS One 8:e75983 (2013).
Ali, M. F., Driscoll, C. B., Walters, P. R., Limper, A. H. & Carmona, E. M. ß-Glucan-Activated Human B Lymphocytes Participate in Innate Immune Responses by Releasing Proinflammatory Cytokines and Stimulating Neutrophil Chemotaxis. J Immunol 195:5318–5326 (2015).
Lee, N. R. et al. House dust mite allergen suppresses neutrophil apoptosis by cytokine release via PAR2 in normal and allergic lymphocytes. Immunol Res 64: 123–132 (2016).
Scheel-Toellner, D. et al. Early events in spontaneous neutrophil apoptosis. Biochem Soc Trans 32:461–464 (2004).
Monteseirín, J. Neutrophils and asthma. J Investig Allergol Clin Immunol 19:340–354 (2009).
Yang, E. J., Choi, E., Ko, J., Kim, D. H. & Lee, J. S. Differential effect of CCL2 on constitutive neutrophil apoptosis between normal and asthmatic subjects. J Cell Physiol 227:2567–2577 (2012).
Kim, E. H. et al. Regulation of Constitutive Neutrophil Apoptosis Due to House Dust Mite Allergen in Normal and Allergic Rhinitis Subjects. PLoS One 9:e105814 (2014).
Kim, D. H. et al. House Dust Mite Allergen Regulates Constitutive Apoptosis of Normal and Asthmatic Neutrophils via Toll-Like Receptor 4. PLoS One 10: e0125983 (2015).
Burrows, K. E. et al. OX40 blockade inhibits house dust mite driven allergic lung inflammation in mice and in vitro allergic responses in humans. Eur J Immunol 45:1116–1128 (2015).
Davidson, C. E. et al. Proteinase-activated receptor-2 activation participates in allergic sensitization to house dust mite allergens in a murine model. Clin Exp Allergy 43:1274–1285 (2013).
de Boer, J. D. et al. Protease-activated receptor-2 deficient mice have reduced house dust mite-evoked allergic lung inflammation. Innate Immun 20:618–625 (2014).
Zheng, J. et al. Suppression of connexin 26 is related to protease-activated receptor 2-mediated pathway in patients with allergic rhinitis. Am J Rhinol Allergy 26: e5–9 (2012).
Post, S. et al. Protease-activated receptor-2 activation contributes to house dust mite-induced IgE responses in mice. PLoS One 9:e91206 (2014).
Kato, T. et al. Mite serine protease activates proteaseactivated receptor-2 and induces cytokine release in human keratinocytes. Allergy 64:1366–1374 (2009).
Shi, J. et al. Induction of IL-6 and IL-8 by house dust mite allergen Der p1 in cultured human nasal epithelial cells is associated with PAR/PI3K/NFkappaB signaling. ORL J Otorhinolaryngol Relat Spec 72:256–265 (2010).
Author information
Authors and Affiliations
Corresponding authors
Electronic supplementary material
Rights and permissions
About this article
Cite this article
Lee, N.R., Lee, JS., Baek, S.Y. et al. Effect of house dust mite on neutrophil apoptosis by cytokine secretion in lymphocytes. Mol. Cell. Toxicol. 12, 371–379 (2016). https://doi.org/10.1007/s13273-016-0041-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13273-016-0041-y