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Identification of aberrant DNA methylation involved in chemoradiation-resistant HCT116 cells via methylation-specific microarray

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Abstract

Preoperative 5-fluorouracil (5-FU) based chemoradiation is regarded as the standard treatment for locally advanced colorectal cancer (CRC). However, advances in using 5-FU in chemoradiotherapy treatment of CRC are limited by the resistant to the chemoradiotherapy. It has been demonstrated that aberrant methylation contributes to radioresistance in a human colorectal tumor cell line. So we analyzed the differential DNA methylation patterns by methylationspecific microarray in a 5-FU-based concurrent chemoradiotherapy resistance cell model which was established by using human CRC cell line HCT116. As a result, we detected a total of 3719 hypomethylated sites and 27940 hypermethylated sites in CRR cells compared to the control. Furthermore, we analyzed top 10 GO terms involved in significantly differential DNA methylation patterns resulted from the 5-FU-based CRR through GO analyses. To further explore the molecular basis of 5-FU-based CRR in CRC cells, CYP1B1 and CYP2C9 genes expression level were shown down regulated by qRT-PCR. These findings might provide a novel insight for chemoradiation resistance in CRC and provide a potential therapy method in CRC patients.

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Authors and Affiliations

  1. Department of Radiation Oncology, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University, Kunming, China

    Wei Xiong & Ting Chen

  2. Department of Colorectal Surgery, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University, Kunming, China

    Yun-Feng Li, Hong-Tao Zhang & Zhi-Bin Yang

  3. Department of Integrated Chinese and Western Medicine, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University, Kunming, China

    Shan Liu

  4. Department of Medical Imaging, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University, Kunming, China

    Ying-Ying Ding, De-Pei Gao & Guan-Shun Wang

  5. Department of Medical Oncology, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University, Kunming, China

    Jian Dong

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  1. Wei Xiong
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Correspondence to Jian Dong.

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These authors contributed equally to this work.

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13273_2016_39_MOESM1_ESM.pdf

Identification of aberrant DNA methylation involved in chemoradiation-resistant HCT116 cells via methylation-specific microarray

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Xiong, W., Li, YF., Liu, S. et al. Identification of aberrant DNA methylation involved in chemoradiation-resistant HCT116 cells via methylation-specific microarray. Mol. Cell. Toxicol. 12, 345–357 (2016). https://doi.org/10.1007/s13273-016-0039-5

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  • DOI: https://doi.org/10.1007/s13273-016-0039-5

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