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Effect of cadmium exposure on antioxidant enzyme catalase in different tissues of Acrossocheilus fasciatus

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Abstract

Catalase (CAT) is an essential antioxidant in organisms, its can eliminate hydrogen peroxides produced in the cellular environment. Previous studies have focused mainly on the functions of CAT by pathogen challenge, but the functions of CAT by metals stress remain unclear. In order to investigate the function of CAT in Acrossocheilus fasciatus (AfCat), we cloned CAT complete cDNA sequences and analyzed its functions by cadmium (Cd) stress. qPCR analysis illustrated that the expression of CAT changed rapidly and dynamically in response to Cd from 0.05 mg L-1 to 1.25 mg L-1. Enzyme activity assay result showed that CAT activity was up-regulated significantly in response to Cd from 0.05 mg L-1 to 1.25 mg L-1. Histochemistry analysis showed that the structure of liver, testis and kidney were severely damaged by exposed to Cd of 1.25 mg L-1. Collectively, the results of our study indicate that AfCat may play an indispensable role in maintaining redox balance under Cd exposure, but AfCat expression will be inhibited when Cd accumulation reaches to the critical concentration. This study will illuminate the potential functions of AfCat in the defense mechanisms of reproductive and metabolic systems under Cd exposure in A. fasciatus.

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Authors and Affiliations

  1. School of Marine Sciences, Ningbo University, Ningbo, Zhejiang, 315211, People’s Republic of China

    Yong-Qiang Zhao, Guo-Di Liu, Cong-Cong Hou, Ying-Li Han & Jun-Quan Zhu

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  1. Yong-Qiang Zhao
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  2. Guo-Di Liu
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  3. Cong-Cong Hou
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  4. Ying-Li Han
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  5. Jun-Quan Zhu
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Correspondence to Jun-Quan Zhu.

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Zhao, YQ., Liu, GD., Hou, CC. et al. Effect of cadmium exposure on antioxidant enzyme catalase in different tissues of Acrossocheilus fasciatus . Mol. Cell. Toxicol. 12, 255–263 (2016). https://doi.org/10.1007/s13273-016-0030-1

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  • DOI: https://doi.org/10.1007/s13273-016-0030-1

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