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Identification of potential biomarkers for xylene exposure by microarray analyses of gene expression and methylation

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Abstract

Xylene is volatile organic compound that has been reported to increase the incidence of cancer and various diseases related to the immune system, cardiovascular systems, respiratory and reproductive organs. However, there are currently few biomarkers in human cases. Using microarray, we analysed 10 participants for the xylene-exposure group and 10 controls that were not exposed to xylene. The two groups were compared in terms of expression levels and methylation patterns. We identified 6 genes that were down-regulated and hyper-methylated, and 132 that were up-regulated and hypo-methylated in the xylene- exposure group compared to control. We sorted out and 28 biomarker candidates were chosen using DAVID. And then, we used IPA to select the significant potential biomarkers in them. We used network analysis and selected 6 significant genes, and these 6 genes showed altered expression and methylation in xylene-exposure group, suggesting that they are suitable potential biomarkers for xylene exposure.

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Authors and Affiliations

  1. Department of Bio-Nanotechnology, Hanyang University, Sangnok-gu, Ansan, Gyeonggi-do, Korea

    Seol Young Kim, Ji Young Hong, Jeong Jin Ahn, Youngjoo Kim & Seung Yong Hwang

  2. Department of Molecular & Life Science, Hanyang University, Sangnok-gu, Ansan, Gyeonggi-do, Korea

    So-Yeon Yu, Gi Won Kim & Seung Yong Hwang

  3. Department of Dermatology, Korea University Medical Center, Seoul, Korea

    Sang Wook Son

  4. Department of Occupational and Environmental Medicine, Korea University Ansan Hospital, Korea University College of Medicine, Danwon-gu, Ansan, Gyeonggi-do, Korea

    Jong-Tae Park

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  1. Seol Young Kim
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  2. Ji Young Hong
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  3. So-Yeon Yu
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  4. Gi Won Kim
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  6. Youngjoo Kim
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  9. Seung Yong Hwang
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Correspondence to Seung Yong Hwang.

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Kim, S.Y., Hong, J.Y., Yu, SY. et al. Identification of potential biomarkers for xylene exposure by microarray analyses of gene expression and methylation. Mol. Cell. Toxicol. 12, 15–20 (2016). https://doi.org/10.1007/s13273-016-0003-4

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  • DOI: https://doi.org/10.1007/s13273-016-0003-4

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