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Inhibition of lewis lung cancer cell growth and migration by fucoidan

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Abstract

Fucoidan has known anticancer activity in various cancer cell types. In the present study, the anticancer activity of fucoidan in Lewis lung cancer cells (LLC) and the underlying mechanism of action were investigated. To explore the mechanism of these anticancer effects, the phosphoinositide 3-kinase (PI3K)-Akt-mammalian target of rapamycin (mTOR) and the apoptosis signaling pathway were examined by western blot analysis. LLC growth was significantly inhibited following treatment with fucoidan (50 μg/mL). Moreover, fucoidan inhibited the invasion and migration of LLCs by regulating matrix metallopeptidase-2 expression. Fucoidan induced down regulation of the PI3K-Akt-mTOR pathway in LLC and caused significant apoptosis through increased mTOR-associated cleaved-caspase-3 expression. Collectively, these findings identify the regulation of PI3K-Akt-mTOR signaling, MMP-2 expression, and caspase-3 activation by fucoidan as a critical anti-cancer mechanism in lung cancer cells, suggesting that fucoidan is a potential therapeutic agent for treating lung cancer.

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Authors and Affiliations

  1. Medical Science Research Institute, Soonchunhyang University Seoul Hospital, Seoul, 336-745, Korea

    Yong-seok Han & Sang Hun Lee

  2. Department of Medical Bioscience, Soonchunhyang University, Asan, 336-745, Korea

    Yong-seok Han & Sang Hun Lee

  3. Laboratory for Vascular Medicine & Stem Cell Biology, Medical Research Institute, Department of Physiology, School of Medicine, Pusan National University, Yangsan, 626-870, Korea

    Jun Hee Lee

  4. Soonchunhyang Medical Science Research Institute, Soonchunhyang University Bucheon Hospital, Bucheon, 420-767, Korea

    Hun Soo Chang

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  1. Yong-seok Han
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  2. Jun Hee Lee
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  3. Hun Soo Chang
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  4. Sang Hun Lee
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Correspondence to Sang Hun Lee.

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These authors equally contributed to this work

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Han, Ys., Lee, J.H., Chang, H.S. et al. Inhibition of lewis lung cancer cell growth and migration by fucoidan. Mol. Cell. Toxicol. 10, 269–276 (2014). https://doi.org/10.1007/s13273-014-0030-y

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  • DOI: https://doi.org/10.1007/s13273-014-0030-y

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