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Molecular & Cellular Toxicology

, Volume 9, Issue 1, pp 67–74 | Cite as

Effect of the size and surface charge of silica nanoparticles on cutaneous toxicity

  • Yoon-Hee Park
  • Hyun Cheol Bae
  • Yeonsue Jang
  • Sang Hoon Jeong
  • Ha Na Lee
  • Woo-In Ryu
  • Min Gun Yoo
  • Yu-Ri Kim
  • Meyoung-Kon Kim
  • Jong Kwon Lee
  • Jayoung Jeong
  • Sang Wook Son
Original Paper

Abstract

Silica nanoparticles (NPs) are widely applied in many fields, such as chemical industry, medicine, cosmetics, and agriculture. However, the hazardous effects of silica NPs exposure are not completely understood. In this study, the two different sizes (20 nm and 100 nm) and different charges (negatively charged [NC] and weakly negatively charged [WNC]) of silica NPs were used. The present study investigated the cytotoxicity and reactive oxygen species (ROS) generation of silica NPs on keratinocytes. The phototoxicity test of silica NPs was performed on skin fibroblast cells. In addition, skin irritation and skin sensitization of silica NPs were studied on HSEM and mouse skin, respectively. The cell viability of NC 20 nm silica NPs was decreased. However, there are no cytotoxicity for NC 100 nm silica NPs and WNC silica NPs (20 and 100 nm). The results for silica NPs-induced ROS generation are consistent with the cytotoxicity test by silica NPs. Further, NC and WNC silica NPs induced no phototoxicity, acute cutaneous irritation, or skin sensitization. These results suggested that silica NPs-induced ROS generation was the determinant of cytotoxicity. This study showed that the smaller size (20 nm) of silica NPs had more toxicity than the larger size (100 nm) of silica NPs for NC silica NPs. Moreover, we observed an effect of surface charge in cytotoxicity and ROS generation, by showing that the NC silica NPs (20 nm) had more toxic than the WNC silica NPs (20 nm). These findings suggested that the surface charge of silica NPs might be the important parameter for silica NPs-induced toxicity. Further study is needed to assess the effect of surface modification of nanotoxicity.

Keywords

Silica Phototoxicity Skin irritation Skin sensitization 

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Copyright information

© The Korean Society of Toxicogenomics and Toxicoproteomics and Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Yoon-Hee Park
    • 1
  • Hyun Cheol Bae
    • 1
  • Yeonsue Jang
    • 1
  • Sang Hoon Jeong
    • 1
  • Ha Na Lee
    • 1
  • Woo-In Ryu
    • 1
  • Min Gun Yoo
    • 1
  • Yu-Ri Kim
    • 2
  • Meyoung-Kon Kim
    • 2
  • Jong Kwon Lee
    • 3
  • Jayoung Jeong
    • 3
  • Sang Wook Son
    • 1
  1. 1.Laboratory of Cell Signaling and Nanomedicine, Department of Dermatology and Division of Brain Korea 21 Project for Biomedical ScienceKorea University College of MedicineSeoulKorea
  2. 2.Department of Biochemistry & Molecular BiologyKorea University College of MedicineSeoulKorea
  3. 3.Toxicological Research DivisionNational Institute of Food and Drug Safety Evaluation, Osong-eupChungchungbuk-doKorea

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