Exposure to UVB radiation induced skin damage that results to increase risk of skin cancer. Despite the clinical importance of skin-induced damage, antioxidants imposed limited therapeutic success. Hydrogen molecule (H2) has been known as a safe antioxidant in the prevention and therapeutic approach towards several diseases. Drinking hydrogen reduced water (HRW), inhalation of hydrogen gas, and injecting H2-dissolved saline are widely accepted to incorporate H2 in the body. However, there is no document about the beneficial effect of hydrogen water bath. Here, we investigated the effect of hydrogen bathing on the UVB-induced skin damage in hairless mice. For this, mice of the bathing group are allowed to freely swim on HRW, and let the HRW penetrate for 60 mins. Scoring of skin injury, reactive oxygen species (ROS) enzyme activity quantification, cytokine analysis, and ultrastructural change of corneocytes were measured after exposure to UVB radiation of 360–540 mJ/cm2. In summary, the bathing with HRW significantly reduced the levels of skin damage, as well as increased activity of glutathione peroxidase. Further, the effect of HRW on cytokine network in the skin after UVB exposure revealed that HRW significantly decreased the level of inflammatory cytokines such as IL-1β, IL-6, TNF-α and IFN-γ. Finally, scanning electron microscopy data revealed low number of defected corneocytes and ultrastructural changes, suggesting that HRW bathing would protect UV-induced cell damage.
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Ichihashi, M. et al. UV-induced skin damage. Toxicology 189:21–39 (2003).
Placzek, M. et al. Ultraviolet B-Induced DNA damage in human epidermis is modified by the antioxidants ascorbic acid and D-á-Tocopherol. J Invest Dermatol 124:304–307 (2005).
Ohta, S. Recent progress toward hydrogen medicine: Potential of molecular hydrogen for preventive and therapeutic applications. Curr Pharm Des 17:2241–2252 (2011).
Ohsawa, I. et al. Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radical. Nat Med 13:688–694 (2007).
Hanaoka, K. et al. The mechanism of the enhanced antioxidant effects against superoxide anion radicals of reduced water produced by electrolysis. Biophys Chem 107:71–82 (2004).
Huang, K. C. et al. Reduced hemodialysis-induced oxidative stress in end-stage renal disease patients by electrolyzed reduced water. Kidney Int 64:704–714 (2003).
Dell’oste, V. et al. Altered expression of UVB-induced cytokines in human papillomavirus. J Gen Virol 89:2461–2466 (2008).
Liebel, F. et al. Irradiation of skin with visible light induces reactive oxygen species and matrix-degrading enzymes. J Invest Dermatol 132:1901–1907 (2012).
Godar, D. E., Miller, S. A. & Thomas, D. P. Immediate and delayed apoptotic cell death mechanisms: UVA versus UVB and UVC radiation. Cell Death Differ 1:59–66 (1994).
Yoon, K. S. et al. Histological study on the effect of electrolyzed reduced water-bathing on UVB radiationinduced skin injury in hairless mice. Biol Pharm Bull 34:1671–1677 (2011).
Xie, K. et al. Hydrogen gas improves survival rate and organ damage in zymosan-induced generalized inflammation model. Shock 34:495–501 (2010).
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Ignacio, R.M., Yoon, Y., Sajo, M.E.J. et al. The balneotherapy effect of hydrogen reduced water on UVB-mediated skin injury in hairless mice. Mol. Cell. Toxicol. 9, 15–21 (2013). https://doi.org/10.1007/s13273-013-0003-6
- Hydrogen molecule
- Hydrogen reduced water
- Skin damage