Abstract
Background
The endangered longhorn beetle Callipogon (Eoxenus) relictus, which was designated as a natural monument since 1968 in Korea is still attracting public concern because of its extraordinary body size. Although mitochondrial genome data of this species was reported using Korean individual in 2017, start codon of cox1 is controversial and the secondary structures of transfer RNAs have not been illustrated.
Objective
To report complete mitochondrial genome of Callipogon (Eoxenus) relictus from Chinese breed.
Methods
We used dissected muscle tissues from an adult of Callipogon (Eoxenus) relictus. A total of 19,276,266,645 bp from 127,657,395 reads were generated. The raw reads were assembled to mitochondrial genome data and annotated. Folded structures of transfer RNAs were drawn. Phylogenetic relationships were estimated by maximum likelihood and Bayesian inference analyses.
Results
The mitochondrial genome of C. relictus was 15,745 bp in length and composed of 37 genes including 13 protein-coding genes (PCGs), two ribosomal RNAs, and 22 transfer RNAs. The overall base composition was 38.40% for A, 30.98% for T, 11.06% for G, and 19.56% for C. Most transfer RNAs were folded into the typical clover-leaf structure except trnS1. Phylogenetic analyses confirmed the monophyletic status of each subfamily.
Conclusion
Mitochondrial genome composition was consistent with previous research, however, we suggest another start codon of cox1 gene and provide illustrated secondary structures of transfer RNAs. Phylogenetic analyses showed that subfamilies Cerambycinae and Prioninae are closely related.
Similar content being viewed by others
References
Ashman LG, Shin S, Zwick A, Ślipiński A, McKenna DD (2022) The first phylogeny of Australasian Lamiinae longhorn beetles (Coleoptera: Cerambycidae) reveals poor tribal classification and a complex biogeographic history. Syst Entomol 47:213–230. https://doi.org/10.1111/syen.12526
Audinet-Serville JG (1832) Nouvelle classification de la famille des longieomes. Ann Soc Entomol Fr 1:118–201
Bernt M, Donath A, Juhling F, Externbrink F, Florentz C, Fritzsch G, Putz J, Middendorf M, Stadler PF (2013) MITOS: improved de novo metazoan mitochondrial genome annotation. Mol Phylogenet Evol 69:313–319. https://doi.org/10.1016/j.ympev.2012.08.023
Cameron SL (2014) How to sequence and annotate insect mitochondrial genomes for systematic and comparative genomics research. Syst Entomol 39:400–411. https://doi.org/10.1111/syen.12071
Chan PP, Lowe TM (2019) tRNAscan-SE: searching for tRNA genes in genomic sequences. Methods Mol Biol 1962:1–14. https://doi.org/10.1007/978-1-4939-9173-0_1
Drumont A, Komiya Z (2010) Subfamily Prioninae Latreille. In: Löbl I, Smetana A (eds) Catalogue of Palaearctic Coleoptera, Volume 6. Chrysomoeloidea. Apollo Books, Stenstrup, p 89
Grant JR, Stothard P (2008) The CGView server: a comparative genomics tool for circular genomes. Nucleic Acids Res 36:W181–W184. https://doi.org/10.1093/nar/gkn179
Greiner S, Lehwark P, Bock R (2019) OrganellarGenomeDRAW (OGDRAW) version 1.3.1: expanded toolkit for the graphical visualization of organellar genomes. Nucleic Acids Res 47:W59–W64. https://doi.org/10.1093/nar/gkz238
Jin JJ, Yu WB, Yang JB, Song Y, dePamphilis CW, Yi TS, Li DZ (2020) GetOrganelle: a fast and versatile toolkit for accurate de novo assembly of organelle genomes. Genome Biol 21:1–31. https://doi.org/10.1186/s13059-020-02154-5
Katoh K, Standley DM (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol 30:772–780. https://doi.org/10.1093/molbev/mst010
Lanfear R, Frandsen PB, Wright AM, Senfeld T, Calcott B (2017) PartitionFinder 2: new methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. Mol Biol Evol 34:772–773. https://doi.org/10.1093/molbev/msw260
Lim J, Yi DK, Kim YH, Lee WH, Kim S, Kang JH, Kim IK (2017) Complete mitochondrial genome of Callipogon relictus Semenov (Coleoptera: Cerambycidae): a natural monument and endangered species in Korea. Mitochondrial DNA B: Resour 2:629–631. https://doi.org/10.1080/23802359.2017.1372718
Monné ML, Monné MA, Mermudes JRM (2009) Inventário das espécies de Cerambycinae (Insecta, Coleoptera, Cerambycidae) do Parque Nacional do Itatiaia, RJ, Brasil. Biota Neotrop 9:283–312. https://doi.org/10.1590/S1676-06032009000300027
Nguyen LT, Schmidt HA, von Haeseler A, Minh BQ (2015) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Mol Biol Evol 32:268–274. https://doi.org/10.1093/molbev/msu300
Nie R, Vogler AP, Yang XK, Lin M (2020) Higher-level phylogeny of longhorn beetles (Coleoptera: Chrysomeloidea) from mitochondrial genomes. Syst Entomol 46:56–70. https://doi.org/10.1111/syen.12447
Pu DQ, Liu HL, Wu XL, Chen ZT (2022) Complete mitochondrial genomes and phylogenetic positions of two longicorn beetles, Anoplophora glabripennis and Demonax pseudonotabilis (Coleoptera: Cerambycidae). Genes 13:1881. https://doi.org/10.3390/genes13101881
Reuter JS, Mathews DH (2010) RNAstructure: software for RNA secondary structure prediction and analysis. BMC Bioinform 11:129. https://doi.org/10.1186/1471-2105-11-129
Ronquist F, Teslenko M, Van Der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: efficient bayesian phylogenetic inference and model choice across a large model space. Syst Biol 61:539–542. https://doi.org/10.1093/sysbio/sys029
Semenov AP (1899) Callipogon (Eoxenus) relictus sp. n. New species of Cerambycidae in fauna of Russia. Russ J Ent Soc 32:562–580
Turnbow RH, Thomas MC (2002) Family 120. Cerambycidae Leach 1815. In: Arnett RH, Thomas MC, Skelley PE, Frank JH (eds) American beetles, vol 2. Scarabaeoidea through Curculionoidea Boca Raton. CRC Press, Polyphaga, pp 568–601
Acknowledgements
This study was supported by Korea National Arboretum (KNA-22-c-25), Korea Forest Service. This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2020R1A6A1A06046235).
Author information
Authors and Affiliations
Corresponding authors
Ethics declarations
Conflict of interest
Yeon-Jae Choi, Taeyoung Jang, A-Young Kim, Jong-Seok Park, and Sung-Jin Cho declare that they have no conflict of interest.
Additional information
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Choi, YJ., Jang, T., Kim, AY. et al. Complete mitochondrial genome of the Chinese Callipogon (Eoxenus) relictus (Cerambycidae: Prioninae) with phylogenetic analyses. Genes Genom 45, 827–833 (2023). https://doi.org/10.1007/s13258-023-01378-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13258-023-01378-6