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Genetic variation of seed oil characteristics in native Korean germplasm of Perilla crop (Perilla frutescens L.) using SSR markers

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Abstract

Background

In order to maximize the use of valuable native Perilla germplasm in South Korea, knowledge of the Perilla seed oil content and genetic variation among native Perilla germplasm resources is very important for the conservation and development of new Perilla seed oil varieties using the native Perilla germplasm accessions preserved from the Rural Development Administration Genebank (RDA-Genebank) collection from South Korea.

Objectives

In this study, we studied population structure and association mapping to identify Perilla SSR markers (PSMs) associated with the five fatty acid contents and two seed characteristics of the native Korean Perilla germplasm accessions of cultivated var. frutescens of the RDA-Genebank collected in South Korea.

Methods

For an association mapping analysis to find PSMs associated with the five fatty acid contents and two seed characteristics of the Perilla germplasm accessions of cultivated var. frutescens, we evaluated the content of five fatty acids of 280 native Korean Perilla germplasm accessions and used 29 Perilla SSR primer sets to measure the genetic diversity and relationships, population structure, and association mapping of the native Korean Perilla germplasm accessions of the RDA-Genebank collected in South Korea.

Results

Five fatty acids of 280 native Korean Perilla accessions were identified as follows: palmitic acid (PA) (5.30–8.66%), stearic acid (SA) (1.60–4.19%), oleic acid (OA) (9.60–22.5%), linoleic acid (LA) (8.38–25.4%), and linolenic acid (LNA) (52.7–76.4%). In a correlation analysis among the five fatty acids and two seed characteristics of the 280 Perilla accessions, the combinations of PA and SA (0.794**) and SA and OA (0.724**) showed a particularly high positive correlation coefficients compare to other combinations. By using an association analysis of the 29 PSMs and the five fatty acids in the 280 Perilla accessions, we found 17 PSMs (KNUPF1, KNUPF2, KNUPF4, KNUPF10, KNUPF16, KNUPF25, KNUPF26, KNUPF28, KNUPF37, KNUPF55, KNUPF62, KNUPF71, KNUPF74, KNUPF77, KNUPF85, KNUPF89, and KNUPF118) associated with the content of the five fatty acid components and two seed characteristics.

Conclusions

These PSMs are considered to be useful molecular markers related to five fatty acid components and two seed characteristics for selecting accessions from the germplasm accessions of the Perilla crop and their related weedy types through association mapping analysis and marker-assisted selection (MAS) breeding programs.

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Acknowledgements

This study was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2022R1F1A1063300) and the Cooperative Research Program for Agriculture Science & Technology Development (project no. PJ014227032019 and PJ0142272019), Rural Development Administration, Republic of Korea.

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  1. Hyeon Park and Kyu Jin Sa contributed equally to this work.

Authors and Affiliations

  1. Department of Applied Plant Sciences, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, 24341, South Korea

    Hyeon Park, Kyu Jin Sa & Ju Kyong Lee

  2. Interdisciplinary Program in Smart Agriculture, Kangwon National University, Chuncheon, 24341, South Korea

    Hyeon Park & Ju Kyong Lee

  3. National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA, Jeonju, 54874, South Korea

    Sookyeong Lee

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  1. Hyeon Park
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Correspondence to Ju Kyong Lee.

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Hyeon Park declares that he has no conflict of interest. Kyu Jin Sa declares that he has no conflict of interest. Sookyeong Lee declares that she has no conflict of interest. Ju Kyong Lee declares that he has no conflict of interest.

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Park, H., Sa, K.J., Lee, S. et al. Genetic variation of seed oil characteristics in native Korean germplasm of Perilla crop (Perilla frutescens L.) using SSR markers. Genes Genom 44, 1159–1170 (2022). https://doi.org/10.1007/s13258-022-01289-y

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