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CALM1 rs3179089 polymorphism might contribute to coronary artery disease susceptibility in Chinese male: a case–control study

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Abstract

Background

Calmodulin 1 (CALM1) mutations are involved in the development of coronary artery disease (CAD). However, the relationship of CALM1 rs3179089 polymorphism with CAD is unknown.

Objective

This study aimed to identify the relationship of CALM1 rs3179089 polymorphism with CAD susceptibility, CALM1 expression, blood pressure, blood glucose, blood coagulation and serum lipid levels of CAD patients.

Methods

550 CAD patients and 550 control subjects were genotyped for CALM1 using Sequenom MassARRAY technology. CALM1 expression level was measured by quantitative real time polymerase chain reaction (qRT-PCR).

Results

CALM1 mRNA expression was higher in CAD patients than that in control subjects (P < 0.001). CAD patients with CC genotype had higher CALM1 mRNA expression level than control subjects with CC genotype (P = 0.006). Genotypic frequency of rs3179089 was different between male patients of CAD and control subjects (P = 0.045). Rs3179089 polymorphism was related to CAD risk of males in recessive model (P = 0.039). Moreover, rs3179089 polymorphism was associated with systolic blood pressure (SBP), diastolic blood pressure (DBP), fasting plasma glucose (FPG), and D-Dimer (D-D) level of patients with CAD in recessive model (P = 0.013 for SBP; P = 0.034 for DBP; P = 0.004 for FPG; P = 0.046 for D-D). In addition, rs3179089 polymorphism was correlated with low-density lipoprotein cholesterol (LDL-C) and total cholesterol (TC) serum levels of patients with CAD in both addictive (P = 0.025 for LDL-C; P = 0.001 for TC) and recessive models (P = 0.001 for LDL-C; P = 0.001 for TC).

Conclusion

CALM1 expression is associated with development of CAD. CALM1 rs3179089 polymorphism affects CAD susceptibility in males, and blood pressure, blood glucose, blood coagulation and serum lipid of CAD patients.

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Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

This work was supported by grants from the National Natural Science Foundation of China (Grant No. 81660741; Grant No. 81573756).

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Author notes

  1. Jingyan Huang, Siyun Huang and Jinhong Li are first co-author.

Authors and Affiliations

  1. The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120, Guangdong, China

    Jingyan Huang

  2. Guangzhou University of Chinese Medicine, Guangzhou, 510405, Guangdong, China

    Jingyan Huang

  3. University at Buffalo, The State University of New York, Buffalo, NY, 14228, USA

    Jingyan Huang

  4. Guangxi University of Chinese Medicine, Nanning, 530299, Guangxi, China

    Jingyan Huang, Siyun Huang, Jinhong Li, Minhua Li, Lin Gong, Tongshun Li & Lian Gu

  5. The First Affiliated Hospital of Guangxi University of Chinese Medicine, No. 89-9 Dongge Road, Nanning, 530023, Guangxi, China

    Siyun Huang, Jinhong Li, Minhua Li, Lin Gong, Tongshun Li & Lian Gu

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  1. Jingyan Huang
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  4. Minhua Li
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  7. Lian Gu
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Contributions

All authors contributed to the study conception and design. This study is supervised by LG. Material preparation, data collection and analysis were performed by JH, SH, JL, ML, LG, TL. The first draft of the manuscript was written by JH and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Lian Gu.

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Conflict of interest

Jingyan Huang, Siyun Huang, Jinhong Li, Minhua Li, Lin Gong, Tongshun Li, Lian Gu declare that they have no competing interests.

Ethics approval

Approval was obtained from the ethics committee of the First Affiliated Hospital of Guangxi University of Chinese Medicine. The procedures used in this study adhere to the tenets of the Declaration of Helsinki.

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Informed consent was obtained from all individual participants included in the study.

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The authors affirm that human research participants provided informed consent for publication of their clinic data.

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Huang, J., Huang, S., Li, J. et al. CALM1 rs3179089 polymorphism might contribute to coronary artery disease susceptibility in Chinese male: a case–control study. Genes Genom 44, 415–423 (2022). https://doi.org/10.1007/s13258-021-01144-6

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  • DOI: https://doi.org/10.1007/s13258-021-01144-6

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