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Propofol enhanced the cell sensitivity to paclitaxel (PTX) in prostatic cancer (PC) through modulation of HOTAIR

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Abstract

Background

PTX is widely used in cancer treatments.

Objective

In this paper, we explored the role and potential molecular mechanism of propofol in regulating PTX sensitivity in PC cells.

Methods

Prostatic cancer cell line PC3 was treated using different concentrations of PTX (10 nM, 50 nM), propofol (150 μM, 300 μM) or transfected with overexpressed HOTAIR plasmid. HOTAIR expression was analyzed by RT-qPCR. Apoptosis of PC3 cells was observed by flow cytometry method while cell viability was evaluated by CCK-8. Moreover, apoptosis-related genes, Bcl-2 and Bax were detected by Western blot methods. E-cadherin, N-cadherin and Vimentin protein concentrations were monitored by ELISA.

Results

PTX significantly increased apoptosis of PC3 cells and reduced cell viability in a dose-dependent manner. Moreover, Protein expression of Bcl-2 was obviously inhibited while Bax protein expression level was provoked. Furthermore, E-cadherin protein concentration increased while N-cadherin and Vimentin decreased due to increasing PTX treatments. HOTAIR expression dropped due to PTX treatment while overexpression of HOTAIR induced cell viability, EMT and deterred apoptosis. Propofol ignited the PTX function while upregulation of HOTAIR partially reversed this.

Conclusion

Propofol enhanced paclitaxel sensitivity in prostatic cancer cells through modulation of HOTAIR in vitro.

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References

  • Braz MG, Braz LG, Mazoti MA, Pinotti MF, Pardini MI, Braz JR, Salvadori DM (2012) Lower levels of oxidative DNA damage and apoptosis in lymphocytes from patients undergoing surgery with propofol anesthesia. Environ Mol Mutagen 53:70–77

    Article  CAS  Google Scholar 

  • Chen K, Rajewsky N (2007) The evolution of gene regulation by transcription factors and microRNAs. Nat Rev Genet 8:93–103

    Article  CAS  Google Scholar 

  • Du Z, Fei T, Verhaak RG, Su Z, Zhang Y, Brown M, Chen Y, Liu XS (2013) Integrative genomic analyses reveal clinically relevant long noncoding RNAs in human cancer. Nat Struct Mol Biol 20:908–913

    Article  CAS  Google Scholar 

  • Glen JB, Hunter SC, Blackburn TP, Wood P (1985) Interaction studies and other investigations of the pharmacology of propofol ('Diprivan’). Postgrad Med J 61(Suppl 3):7–14

    CAS  PubMed  Google Scholar 

  • Gupta RA, Shah N, Wang KC, Kim J, Horlings HM, Wong DJ, Tsai MC, Hung T, Argani P, Rinn JL et al (2010) Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis. Nature 464:1071–1076

    Article  CAS  Google Scholar 

  • Iyer MK, Niknafs YS, Malik R, Singhal U, Sahu A, Hosono Y, Barrette TR, Prensner JR, Evans JR, Zhao S et al (2015) The landscape of long noncoding RNAs in the human transcriptome. Nat Genet 47:199–208

    Article  CAS  Google Scholar 

  • Jian L, Xie LP, Lee AH, Binns CW (2004) Protective effect of green tea against prostate cancer: a case-control study in southeast China. Int J Cancer 108:130–135

    Article  CAS  Google Scholar 

  • Jiang J, Wang S, Wang Z, Cai J, Han L, Xie L, Han Q, Wang W, Zhang Y, He X et al (2020b) HOTAIR promotes paclitaxel resistance by regulating CHEK1 in ovarian cancer. Cancer Chemother Pharmacol 86:295–305

    Article  CAS  Google Scholar 

  • Jiang J, Wang S, Wang Z, Cai J, Han L, Xie L, Han Q, Wang W, Zhang Y, He X et al (2020a) HOTAIR promotes paclitaxel resistance by regulating CHEK1 in ovarian cancer. Cancer Chemother Pharmacol 11:123–134

    Google Scholar 

  • Kamangar F, Dores GM, Anderson WF (2006) Patterns of cancer incidence, mortality, and prevalence across five continents: defining priorities to reduce cancer disparities in different geographic regions of the world. J Clin Oncol 24:2137–2150

    Article  Google Scholar 

  • Kothari AN, Mi Z, Zapf M, Kuo PC (2014) Novel clinical therapeutics targeting the epithelial to mesenchymal transition. Clin Transl Med 3:35

    Article  Google Scholar 

  • Li CP, Qiu GZ, Liu B, Chen JL, Fu HT (2016) Neuroprotective effect of lignans extracted from Eucommia ulmoides Oliv. on glaucoma-related neurodegeneration. Neurol Sci 37:755–762

    Article  Google Scholar 

  • Ling Z, Wang X, Tao T, Zhang L, Guan H, You Z, Lu K, Zhang G, Chen S, Wu J et al (2017) Involvement of aberrantly activated HOTAIR/EZH2/miR-193a feedback loop in progression of prostate cancer. J Exp Clin Cancer Res 36:159

    Article  Google Scholar 

  • Qian J, Shen S, Chen W, Chen N (2018) Propofol reversed hypoxia-induced docetaxel resistance in prostate cancer cells by preventing epithelial-mesenchymal transition by inhibiting hypoxia-inducible factor 1α. Biomed Res Int 8:4174232

    Google Scholar 

  • Rinn JL, Kertesz M, Wang JK, Squazzo SL, Xu X, Brugmann SA, Goodnough LH, Helms JA, Farnham PJ, Segal E et al (2007) Functional demarcation of active and silent chromatin domains in human HOX loci by noncoding RNAs. Cell 129:1311–1323

    Article  CAS  Google Scholar 

  • Shang Y (2018) LncRNA THOR acts as a retinoblastoma promoter through enhancing the combination of c-myc mRNA and IGF2BP1 protein. Biomed Pharmacother 106:1243–1249

    Article  CAS  Google Scholar 

  • Shang Z, Feng H, Cui L, Wang W, Fu H (2018) Propofol promotes apoptosis and suppresses the HOTAIR-mediated mTOR/p70S6K signaling pathway in melanoma cells. Oncol Lett 15:630–634

    PubMed  Google Scholar 

  • Shetti D, Zhang B, Fan C, Mo C, Lee BH, Wei K (2019) Low dose of paclitaxel combined with XAV939 attenuates metastasis, angiogenesis and growth in breast cancer by suppressing wnt signaling. Cells 8:19–23

    Article  Google Scholar 

  • Siegel RL, Miller KD, Jemal A (2017) Cancer statistics, 2017. CA Cancer J Clin 67:7–30

    Article  Google Scholar 

  • Sobue S, Mizutani N, Aoyama Y, Kawamoto Y, Suzuki M, Nozawa Y, Ichihara M, Murate T (2016) Mechanism of paclitaxel resistance in a human prostate cancer cell line, PC3-PR, and its sensitization by cabazitaxel. Biochem Biophys Res Commun 479:808–813

    Article  CAS  Google Scholar 

  • Soltani-Sedeh H, Irani S, Mirfakhraie R, Soleimani M (2019) Potential using of microRNA-34A in combination with paclitaxel in colorectal cancer cells. J Cancer Res Ther 15:32–37

    CAS  PubMed  Google Scholar 

  • Swain SM, Honig SF, Tefft MC, Walton L (1995) A phase II trial of paclitaxel (Taxol) as first line treatment in advanced breast cancer. Invest New Drugs 13:217–222

    Article  CAS  Google Scholar 

  • Tang Q, Hann SS (2018) HOTAIR: an oncogenic long non-coding RNA in human cancer. Cell Physiol Biochem 47:893–913

    Article  CAS  Google Scholar 

  • Wang P, Chen J, Mu LH, Du QH, Niu XH, Zhang MY (2013) Propofol inhibits invasion and enhances paclitaxel- induced apoptosis in ovarian cancer cells through the suppression of the transcription factor slug. Eur Rev Med Pharmacol Sci 17:1722–1729

    CAS  PubMed  Google Scholar 

  • Wang H, Qin R, Guan A, Yao Y, Huang Y, Jia H, Huang W, Gao J (2018) HOTAIR enhanced paclitaxel and doxorubicin resistance in gastric cancer cells partly through inhibiting miR-217 expression. J Cell Biochem 119:7226–7234

    Article  CAS  Google Scholar 

  • Wani MC, Taylor HL, Wall ME, Coggon P, McPhail AT (1971) Plant antitumor agents. VI. The isolation and structure of taxol, a novel antileukemic and antitumor agent from Taxus brevifolia. J Am Chem Soc 93:2325–2327

    Article  CAS  Google Scholar 

  • Weaver BA (2014) How Taxol/paclitaxel kills cancer cells. Mol Biol Cell 25:2677–2681

    Article  Google Scholar 

  • Wu KC, Yang ST, Hsia TC, Yang JS, Chiou SM, Lu CC, Wu RS, Chung JG (2012) Suppression of cell invasion and migration by propofol are involved in down-regulating matrix metalloproteinase-2 and p38 MAPK signaling in A549 human lung adenocarcinoma epithelial cells. Anticancer Res 32:4833–4842

    CAS  PubMed  Google Scholar 

  • Xu WB, Hou JQ, Chang JK, Li S, Liu GC, Cao SQ (2019) The mechanism of HOTAIR regulating the proliferation and apoptosis of prostate cancer cells by targeting down-regulation of miR-152 to improve the expression of FOXR2. Zhonghua Yi Xue Za Zhi 99:1887–1892

    CAS  PubMed  Google Scholar 

  • Xue X, Yang YA, Zhang A, Fong KW, Kim J, Song B, Li S, Zhao JC, Yu J (2016) LncRNA HOTAIR enhances ER signaling and confers tamoxifen resistance in breast cancer. Oncogene 35:2746–2755

    Article  CAS  Google Scholar 

  • Xue YN, Yu BB, Liu YN, Guo R, Li JL, Zhang LC, Su J, Sun LK, Li Y (2019) Zinc promotes prostate cancer cell chemosensitivity to paclitaxel by inhibiting epithelial-mesenchymal transition and inducing apoptosis. Prostate 79:647–656

    Article  CAS  Google Scholar 

  • Zhang JJ, Zhou XH, Zhou Y, Wang YG, Qian BZ, He AN, Shen Z, Hu HY, Yao Y (2019) Bufalin suppresses the migration and invasion of prostate cancer cells through HOTAIR, the sponge of miR-520b. Acta Pharmacol Sin 40:1228–1236

    Article  Google Scholar 

  • Zhu L, Chen L (2019) Progress in research on paclitaxel and tumor immunotherapy. Cell Mol Biol Lett 24:40

    Article  Google Scholar 

  • Zhu Y, Yu RK, Ji AF, Yao XL, Fang JJ, Jin XD (2015) Effects of long non-coding RNA-HOTAIR on the cell cycle and invasiveness of prostate cancer. Zhonghua Nan Ke Xue 21:792–796

    CAS  PubMed  Google Scholar 

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Author notes

  1. Xuesong Yang and Jiao Qin contributed equally to this article.

Authors and Affiliations

  1. Department of Urology, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, China

    Xuesong Yang

  2. Department of Urology, The First Affiliated Hospital of Jinan University, Guangzhou, China

    Xuesong Yang

  3. Department of Anesthesiology, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, China

    Jiao Qin

  4. Department of Urology , West China Fourth Hospital, Sichuan University, Chengdu, Sichuan, China

    Chunyu Gong

  5. Department of Anesthesiology, West China Hospital, Sichuan University, No. 38, Guoxue Ave, Wuhou, Chengdu, Sichuan, 610041, China

    Jing Yang

Authors
  1. Xuesong Yang
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  2. Jiao Qin
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  3. Chunyu Gong
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  4. Jing Yang
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Correspondence to Chunyu Gong or Jing Yang.

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Xuesong Yang, Jiao Qin, Chunyu Gong and Jing Yang declare that they have no conflict of interest.

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This study had been approved by Affiliated hospital of north sichuan medical college. No human or animal was involved in the study.

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Yang, X., Qin, J., Gong, C. et al. Propofol enhanced the cell sensitivity to paclitaxel (PTX) in prostatic cancer (PC) through modulation of HOTAIR. Genes Genom 43, 807–814 (2021). https://doi.org/10.1007/s13258-021-01093-0

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  • DOI: https://doi.org/10.1007/s13258-021-01093-0

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