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Diagnostic and therapeutic biomarkers for Alzheimer’s disease in human-derived platelets

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Abstract

Background

Diagnosis of current Alzheimer’s disease (AD) is difficult even for medical specialists, and there is no clear biomarker. Also, aging is highly related to the onset of AD.

Objectives

The purpose of this study is to screen miRNA as an aging-considered biomarker for AD treatment and diagnosis.

Methods

The patient group for this study was divided into a young normal, old normal, or AD group. We developed a method of discovering sequentially expressed miRNAs to distinguish miRNAs that were sequentially expressed in the three groups.

Results

Sequentially expressed miRNAs correlated highly with the patient’s age, and most showed expression patterns that distinguished young, old, and AD. Specifically, the miRNA expression we found showed similar patterns in the brains of patients with AD. Among the selected miRNAs, one set derived from the same precursor: The expression of miR-150 was a disease- and age-specific downregulation in both 3p and 5p forms, and the precursor also had the same pattern. We named that triple matching. Also, the found miR-150 precursor had AD-specific miRNA-imbalance characteristics.

Conclusions

We developed a novel AD diagnostic method using triple matching and miRNA-imbalance. The triple matching and miRNA imbalance-based relative ratio diagnosis method we developed will be very powerful in resolving the challenges of absolute diagnostic quantification based on biomarker expression. Also, our research results suggest the possibility of a treatment target for AD.

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Acknowledgements

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science, and Technology (2016R1C1B2007025) and by the National Dementia Research and Development Program of the Korea Health Industry Development Institute (KHIDI) funded by the Korean government (Ministry of Health and Welfare) (HI18C1671).

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Authors and Affiliations

  1. Biorchestra Co. Ltd., Techno4-ro 17, Daejeon, 34013, South Korea

    Jae-Woong Min, Dae Hoon Kim, Byeong-Gyu Park & Jin-Hyeob Ryu

  2. Department of Biomedical Laboratory Science, College of Medical Science, Konyang University, Daejeon, 35365, South Korea

    Jina Lee, Hui-Jin Mun & Hyun-Jeong Cho

  3. Department of Neurology, College of Medicine, Konyang University Hospital, Konyang University, Daejeon, 35365, South Korea

    Bora Yoon

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  1. Jae-Woong Min
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Correspondence to Jin-Hyeob Ryu or Hyun-Jeong Cho.

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Jae-Woong Min, Jina Lee, Hui-Jin Mun, Dae Hoon Kim, Byeong-Gyu Park, Bora Yoon, Jin-Hyeob Ryu, and Hyun-Jeong Cho declare that they have no conflicts of interest.

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Min, JW., Lee, J., Mun, HJ. et al. Diagnostic and therapeutic biomarkers for Alzheimer’s disease in human-derived platelets. Genes Genom 42, 1467–1475 (2020). https://doi.org/10.1007/s13258-020-01015-6

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