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Protective mechanism of fdft1 in steroid hormone synthesis pathway in SD rats with acute hypoxic injury

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Abstract

Background

The acute hypoxic injury caused by the plain population entering the plateau in a short period of time has become the main cause of endangering the health of the people who rush into the plateau.

Objective

The study aimed to identify the key genes which participate in resisting the acute hypoxic injury in SD Rats by transcriptomic profile analysis.

Methods

48 Sprague Dawley (SD) male rats were enrolled and randomly divided into four groups (0h, 24h, 48h, 72h) and housed in hypobaric hypoxia chamber with altitude 6000m for different periods of time to make them acute hypoxic injury. The transcriptomic profile of the lung tissue of the rats was analysed by RNA second-generation sequencing combined with bioinformatics analysis.

Results

The results of GO and KEGG function classification analysis revealed that the differential expression genes enriched in steroid hormone synthesis pathway especially in 48h group compared to F0 group. Further analysis revealed that Farnesyl Diphosphate Farnesyl Transferase 1 (fdft1) gene encoding a rate-limiting enzyme in steroid hormone synthesis pathway was significant differently expressed between the groups. The expression levels of fdft1 gene were further verified by RT-PCR and Western-blot methods.

Conclusions

The results suggest that fdft1 gene plays an important role in responding to acute hypoxic injury by regulating steroid hormone biosynthesis.

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Funding

This research was supported by the Second Tibetan Plateau Scientific Expedition and Research Program (STEP), Grant No. 2019QZKK0606, Project 31660254 of the Chinese National Natural Science Foundation, Project 2017-ZJ-Y13 of the Key Laboratory of Medicinal Animal and Plant resources of the Qinghai-Tibetan Plateau, Projects 2019-ZJ-7042 of the Department of Science and Technology of Qinghai Province, Projects 201910743003 of National Students’ Platform for Innovation and Entrepreneurship Training Program.

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Authors and Affiliations

  1. College of Medicine, Qinghai University, Xining, Qinghai, China

    Xue Lin, Haiyan Wang & Xiaoyan Pu

  2. Qinghai Normal University, Xining, Qinghai, China

    Xiaoyan Pu

Authors
  1. Xue Lin
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  2. Haiyan Wang
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  3. Xiaoyan Pu
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Corresponding author

Correspondence to Xiaoyan Pu.

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The authors declare that there is no conflict of interest.

Ethics and consent statement

The animals involved in the study were handled in accordance with the National Regulations on the Administration of Laboratory Animals (GB14923-2010). The animal experiment scheme was approved and examined by the Ethics Committee of School of Medicine of Qinghai University.

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Lin, X., Wang, H. & Pu, X. Protective mechanism of fdft1 in steroid hormone synthesis pathway in SD rats with acute hypoxic injury. Genes Genom 42, 1319–1326 (2020). https://doi.org/10.1007/s13258-020-00999-5

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  • DOI: https://doi.org/10.1007/s13258-020-00999-5

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