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Molecular characterization of Arabidopsis thaliana LSH1 and LSH2 genes

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Abstract

Background

Arabidopsis thaliana genome encodes ten DUF640 (short for domain of unknown function 640)/ALOG (short for Arabidopsis LSH1 and Oryza G1) proteins, also known as light-dependent short hypocotyl (LSH) proteins. While some of the LSH genes regulate organ boundary determination and shade avoidance response, the function of most of these genes remains largely unknown.

Objective

In this study, we aimed to characterize the function of AtLSH1 and AtLSH2 in Arabidopsis.

Methods

We overexpressed AtLSH1 and AtLSH2 (with or without the FLAG tag) in Arabidopsis Col-0 plants under the control of the 35S promoter. We also generated knockout or knockdown lines of these genes by miRNA-induced gene silencing (MIGS). We conducted intensive phenotypic analysis of these transgenic lines, and finally performed RNA-seq analysis of two AtLSH2 overexpression (OX) lines.

Results

Although AtLSH1 and AtLSH2 amino acid sequences showed high similarly, AtLSH2-OX lines showed much higher levels of their transcripts than those of AtLSH1-OX lines. Additionally, overexpression of AtLSH1 and AtLSH2 greatly inhibited hypocotyl elongation in a light-independent manner, and reduced both vegetative and reproductive growth. However, knockout or knockdown of both these AtLSH genes did not affect plant phenotype. Gene Ontology (GO) analysis of differentially expressed genes (DEGs) identified by RNA-seq revealed enrichment of the GO term ‘response to stimulus’, included phytohormone-responsive genes; however, genes responsible for the abnormal phenotypes of AtLSH2-OX lines could not be identified.

Conclusion

Although our data revealed no close association between light and phytohormone signaling components, overexpression of AtLSH1 and AtLSH2 greatly reduced vegetative and reproductive growth of Arabidopsis plants. This property could be used to generate new plants by regulating expression of AtLSH1 and AtLSH2.

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Acknowledgements

This work was supported by a grant from the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2014068885), Republic of Korea.

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Authors and Affiliations

  1. Department of Biological Sciences, College of Biological Science and Biotechnology, Chungnam National University, Daejeon, 34134, Republic of Korea

    Myungjin Lee, Hayong Song, Ju Yeon Yang, Soyun Kim & Yoonkang Hur

  2. School of Agriculture, Yunnam University, Kunming, 650091, China

    Xiangshu Dong

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  1. Myungjin Lee
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  2. Xiangshu Dong
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Correspondence to Yoonkang Hur.

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Lee, M., Dong, X., Song, H. et al. Molecular characterization of Arabidopsis thaliana LSH1 and LSH2 genes. Genes Genom 42, 1151–1162 (2020). https://doi.org/10.1007/s13258-020-00985-x

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