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MicroRNA-377-3p inhibits growth and invasion through sponging JAG1 in ovarian cancer

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Abstract

Background

Ovarian cancer is the one of the most deadly gynecologic malignancy among cancer related death in women. However, the treatment for ovarian cancer is still limited. In this study, we aimed to explore the inhibition potential of miR-377-3p in ovarian cancer and explore the mechanism of this effect.

Methods

Quantitative real-time PCR was used to detect the mRNA or microRNA (miRNA) levels. CCK-8, wound-healing, transwell assay were used to detect cell proliferation, migration and invasion. The protein levels were examined by western blot. The dual luciferase reporter assay was conducted to examine the luciferase activity. Tumor volume was measured and Ki67 was detected via immunohistochemistry.

Results

qRT-PCR results showed that miR-377-3p was downregulated in ovarian cancer patients. MiR-377-3p mimics suppressed cell proliferation, migration, invasion and decreased the JAG1 level. However, miR-377-3p inhibitor promoted these appearances. Interestingly, we found JAG1 was a target gene of miR-377-3p. JAG1 overexpression reversed the miR-377-3p-induced inhibition of proliferation and invasion. In addition, miR-377-3p inhibited ovarian cancer tumorigenesis in vivo, indicating by decreased tumor volume and staining of Ki67.

Conclusion

The results showed that miR-377-3p inhibited growth and invasion of ovarian cancer cells by targeting JAG1.

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Correspondence to Bin Yang.

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Conflict of interest

Liulin Tang, Bin Yang, Xiaolan Cao, Qin Li, Li Jiang and Dan Wang declare that they have no competing interests, and all authors should confirm its accuracy.

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The experimental protocol was obeyed by World Medical Association Declaration of Helsinki. Informed consent was obtained from all individual participants prior to the study.

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Tang, L., Yang, B., Cao, X. et al. MicroRNA-377-3p inhibits growth and invasion through sponging JAG1 in ovarian cancer. Genes Genom 41, 919–926 (2019). https://doi.org/10.1007/s13258-019-00822-w

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  • DOI: https://doi.org/10.1007/s13258-019-00822-w

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