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Expression analysis of miR-221-3p and its target genes in horses

Genes & Genomics volume 41, pages 459–465 (2019)Cite this article

Abstract

Background

A microRNA (miRNA) is a small non-coding RNA (ncRNA) approximately 20 nucleotides long and it affects gene expression through mRNA cleavage or translational repression. Horses (Equus caballus) have been domesticated and bred to enhance their speed for racing. It has been studied extensively with genetic diversity, origins and evolution.

Objectives

We examined expression patterns of miR-221-3p and its target gene CDKN1C in various horse tissues.

Methods

We used bioinformatic tools to examine target gene, seed region and evolutionary conservation of miR-221-3p. The expression patterns of miR-221-3p and its target gene CDKN1C were analyzed by quantitative polymerase chain reaction (qPCR).

Results

Among eight tissues of horse, miR-221-3p was highly expressed in cerebellum and spleen. On the other hand, only medulla was highly expressed in CDKN1C gene.

Conclusion

Our study provides expression data of miR-221-3p and CDKN1C gene in horse and suggests the fundamental information for future studies in relation to functional importance.

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Acknowledgements

This work was supported by a grant from the Ministry of Education, Republic of Korea (NRF-2015R1D1A1A01056650).

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Affiliations

  1. Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan, 46241, Republic of Korea

    So-Won Kim, Ara Jo, Jennifer Im, Hee-Eun Lee & Heui-Soo Kim

  2. Institute of Systems Biology, Pusan National University, Busan, 46241, Republic of Korea

    So-Won Kim, Ara Jo, Hee-Eun Lee & Heui-Soo Kim

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  1. So-Won Kim
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  2. Ara Jo
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  3. Jennifer Im
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  4. Hee-Eun Lee
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Correspondence to Heui-Soo Kim.

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All authors declare that they have no conflicts of interest.

Ethical approval

The animal protocol and sample extraction method in this study were reviewed by the Pusan National University-Institutional Animal Care and Use Committee (PNU-IACUC) for the ethical procedures and scientific care (approval number PNU-2013-0411).

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This article does not contain any studies with human participants performed by any of the authors.

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Kim, SW., Jo, A., Im, J. et al. Expression analysis of miR-221-3p and its target genes in horses. Genes Genom 41, 459–465 (2019). https://doi.org/10.1007/s13258-018-00778-3

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  • DOI: https://doi.org/10.1007/s13258-018-00778-3

Keywords

  • Equus caballus
  • Micro RNA 221-3p
  • CDKN1C
  • Quantitative polymerase chain reaction (qPCR)
  • Expression analysis