Genes & Genomics

, Volume 40, Issue 4, pp 373–379 | Cite as

Genetic analysis assessed by microsatellites for a diallel mating design of two geographical stocks of the blood clam Tegillarca granosa

  • Yinghui Dong
  • Hanhan Yao
  • Xiaolong Zhou
  • Zhihua Lin
Research Article


To determine the potential for productive efficiency and genetic improvement in the blood clam Tegillarca granosa, four offspring populations (ZZ, ZK, KZ and KK) were produced from a diallel mating of two different geographical stocks (Z and K). The levels of genetic diversity and population structures of four populations were analyzed using 14 polymorphic microsatellites. The results showed that the mean observed heterozygosities (Ho) of reciprocal cross populations (ZK and KZ) was higher than those of pure populations(ZZ and KK). The largest values of genetic differentiation coefficient (Fst = 0.067) and Nei’s unbiased genetic distance (Dc = 0.263) were between ZK and KZ, and the smallest (Fst = 0.020, Dc = 0.116) were between ZZ and KK, which revealed that the largest genetic divergence was between the two reciprocal cross populations and the smallest was between two pure populations. This study demonstrated that the reciprocal cross populations of T. granosa had an extensive genetic difference and improvement, which may be advantageous for future breeding studies.


Tegillarca granosa Reciprocal cross Microsatellite Genetic variance 



This research was supported by Modern Agro-industry Technology Research System (Grant No: CARS-48), National Infrastructure of Fishery Germplasm Resources Programme (2015DKA30470) and General project of Zhejiang Provincial Educational Department (Grant No: Y201329410).

Compliance with ethical standards

Conflict of interest

Yinghui Dong declares that he has no conflict of interest. Hanhan Yao declares that she has no conflict of interest. Xiaolong Zhou declares that he has no conflict of interest. Zhihua Lin declares that he has no conflict of interest.

Ethical approval

This article does not contain any studies with human subjects or animal performed by any of the above authors.


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Copyright information

© The Genetics Society of Korea and Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  • Yinghui Dong
    • 1
  • Hanhan Yao
    • 1
  • Xiaolong Zhou
    • 2
  • Zhihua Lin
    • 1
  1. 1.Zhejiang Key Laboratory of Aquatic Germplasm Resources, College of Biological and Environmental SciencesZhejiang Wanli UniversityNingboPeople’s Republic of China
  2. 2.College of Fisheries and Life SciencesShanghai Ocean UniversityShanghaiPeople’s Republic of China

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