Genetic analysis assessed by microsatellites for a diallel mating design of two geographical stocks of the blood clam Tegillarca granosa
To determine the potential for productive efficiency and genetic improvement in the blood clam Tegillarca granosa, four offspring populations (ZZ, ZK, KZ and KK) were produced from a diallel mating of two different geographical stocks (Z and K). The levels of genetic diversity and population structures of four populations were analyzed using 14 polymorphic microsatellites. The results showed that the mean observed heterozygosities (Ho) of reciprocal cross populations (ZK and KZ) was higher than those of pure populations(ZZ and KK). The largest values of genetic differentiation coefficient (Fst = 0.067) and Nei’s unbiased genetic distance (Dc = 0.263) were between ZK and KZ, and the smallest (Fst = 0.020, Dc = 0.116) were between ZZ and KK, which revealed that the largest genetic divergence was between the two reciprocal cross populations and the smallest was between two pure populations. This study demonstrated that the reciprocal cross populations of T. granosa had an extensive genetic difference and improvement, which may be advantageous for future breeding studies.
KeywordsTegillarca granosa Reciprocal cross Microsatellite Genetic variance
This research was supported by Modern Agro-industry Technology Research System (Grant No: CARS-48), National Infrastructure of Fishery Germplasm Resources Programme (2015DKA30470) and General project of Zhejiang Provincial Educational Department (Grant No: Y201329410).
Compliance with ethical standards
Conflict of interest
Yinghui Dong declares that he has no conflict of interest. Hanhan Yao declares that she has no conflict of interest. Xiaolong Zhou declares that he has no conflict of interest. Zhihua Lin declares that he has no conflict of interest.
This article does not contain any studies with human subjects or animal performed by any of the above authors.
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