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Proteomic profiles of Daphnia magna exposed to lead (II) acetate trihydrate and atrazine

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Abstract

In this study, acute toxicity tests were performed to evaluate the impacts of lead (II) acetate trihydrate and atrazine on aquatic organism using a typical freshwater flea, Daphnia magna. Besides, the proteomic profile of the treated D. magna (LC20) was investigated in the pH range from 3 to 10 using 2-dimensional gel electrophoresis method and then analyzed by the Progenesis software to explore the differentially expressed proteins (DEPs) compared to the control organisms. The results showed that there were 179 DEPs caused by these two toxic compounds. More interestingly, the identified DEPs have been found to be involved in energy metabolism, oxidative stress response, dynamic structure of cytoskeleton filaments and effected by two chemicals in opposite ways: up- and down-regulation. A significant difference of protein expression between the control and the stress-exposed D. magna which confirmed the effect of toxic chemicals has been observed. In summary, the proteomic approach as well as the detailed analysis results were proved to be useful for understanding the novel molecular mechanisms for lead or atrazine induced pathological changes. Moreover, specific DEPs can be used as novel biomarker candidates to detect these heavy metal and pesticide.

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Abbreviations

Pb(CH3COO)2.3H2O:

Lead (II) acetate trihydrate

MALDI-TOF:

Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometer

pI:

Isoelectric point

H2O2 :

Hydrogen peroxide

Vtg:

Vitellogenin protein

SOD:

Superoxide dismutase

CAT:

Catalase protein

Tsr:

Twinstar-like protein

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Acknowledgements

This work was conducted with the support of the “Cooperative Research Program for agriculture Science and Technology Development (Project title: Risk Assessment Research and Development of Rapid Diagnostic Method for Biological, Chemical and Environmental Animal Disease, Project No: PJ01052301)” Rural Development Administration, Republic of Korea. The authors are grateful for their support.

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Authors and Affiliations

  1. Department of Bioprocess Engineering, Chonbuk National University, 567 Baekje-daero, Deokjin-Gu Jeonju, Jeonbuk, 54896, South Korea

    Vu Quynh Anh Le & Jiho Min

  2. School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju, 28644, South Korea

    Ji-Young Ahn, Sung-Jin Cho & Yang-Hoon Kim

  3. Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University, 567 Baekje-daero, Deokjin-Gu Jeonju, Jeonbuk, 54896, South Korea

    Mi Young Heo & Jiho Min

  4. National Institute of Animal Science, RDA 114, Sinbang 1-gil, Seonghwan-eup, Seobuk-gu, Cheonan, Chungnam, 31000, South Korea

    Hobaek Yoon & Jihoo Park

  5. College of Veterinary Medicine, Western University of Health Sciences, 309 E Second Street, Pomona, CA, 91766, USA

    Jung Ho Ko, Lyon Lee & Janet Han

  6. Department of Food Science and Biotechnology, Shin Ansan University, 135, Sinansandaehak-ro, Danwon-Gu, Ansan, 425-792, South Korea

    Sang Yong Kim

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  1. Vu Quynh Anh Le
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Correspondence to Yang-Hoon Kim or Jiho Min.

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Conflict of interest

Vu Quynh Anh Le declares that he/she has no conflict of interest. Ji-Young Ahn declares that he/she has no conflict of interest. Mi Young Heo declares that he/she has no conflict of interest. Sung-Jin Cho declares that he/she has no conflict of interest. Hobaek Yoon declares that he/she has no conflict of interest. Jihoo Park declares that he/she has no conflict of interest. Jung Ho Ko declares that he/she has no conflict of interest. Lyon Lee declares that he/she has no conflict of interest. Janet Han declares that he/she has no conflict of interest. Sang Yong Kim declares that he/she has no conflict of interest. Yang-Hoon Kim declares that he/she has no conflict of interest. Jiho Min declares that he/she has no conflict of interest.

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The article does not contain any studies with human participants or animals performed by any of the authors.

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Vu Quynh Anh Le and Ji-Young Ahn have contributed equally to this work.

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Le, V.Q.A., Ahn, JY., Heo, M.Y. et al. Proteomic profiles of Daphnia magna exposed to lead (II) acetate trihydrate and atrazine. Genes Genom 39, 887–895 (2017). https://doi.org/10.1007/s13258-017-0551-6

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  • DOI: https://doi.org/10.1007/s13258-017-0551-6

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