Abstract
In present study, the complete mitochondrial genome of Sinibotia reevesae was first sequenced using the next-generation sequencing technology and annotated using bioinformatic tools. The circular mitochondrial genome was 16,572 bp in length, and contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and 1 displacement loop locus. It presents a typical gene organization and order for completely sequenced cypriniformes mitogenomes. The control region could be divided into three parts included the extended termination associated sequence domain, the central conserved domain and the conserved sequence block. Interestingly, two stem-loop domains were found in control region and OL region, respectively. Furthermore, phylogenetic analyses using concatenated amino acid and nucleotide sequences of the 13 protein-coding genes with two different methods (Maximum likelihood and Bayesian analysis) both highly supported the close relationship of S. reevesae and Sinibotia superciliaris, which was in line with the previous classifications based on morphological and molecular studies. These data provide useful information for a better understanding of the mitogenomic diversities and evolution in fish as well as novel genetic markers for studying population genetics and species identification.
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Acknowledgements
This work was supported by the National Natural Science Funds (No. 31402305), the Educational Commission of Sichuan Province of China (No. KYTD201009; No. 15ZA0285), the department of science and technology of Sichuan Province of China (No. 2015JY0262) and the scientific research project of Neijiang Normal University (No. 16JC08).
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This article does not contain any studies with human subjects by any of the authors. The animal experiment throughout the study was conducted according to the Chinese Ministry of Science and Technology Guiding Directives for Humane Treatment of Laboratory Animals.
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Zou, YC., Xie, BW., Qin, CJ. et al. The complete mitochondrial genome of a threatened loach (Sinibotia reevesae) and its phylogeny. Genes Genom 39, 767–778 (2017). https://doi.org/10.1007/s13258-017-0541-8
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DOI: https://doi.org/10.1007/s13258-017-0541-8