Forensic and population genetic analyses of the GlobalFiler STR loci in the Mongolian population
- 217 Downloads
We have analyzed 24 loci including autosomal and Y-chromosomal short tandem repeats (STRs), Y-indel, and sex-determining marker in a sample of 267 unrelated individuals from the Mongolian population using the GlobalFiler™ PCR Amplification Kit to provide an expanded and more reliable forensic database. Khalkh among 15 Mongolian minor-groups accounts for about 80% of the entire Mongolian population. A total of 267 different DNA profiles were found in this work. The highest gene diversity was observed in the SE33 (0.9376) locus, and the lowest value was found in the TPOX (0.6142) locus. Although individual power of discrimination estimates varied at the studied loci, combined probability of match from the 21 STR loci was estimated to be 1.139 × 10−24, which is highly informative. Based on the results of pairwise F ST genetic distances and multi-dimensional scaling plot showed that Mongolians were clustered into Europeans and Asians, although Mongolia is geographically located in Northeastern Asia. Thus, the present survey of the Mongolian population may help establish a comprehensive reference database for forensic and population genetic analyses.
KeywordsForensic genetics STRs GlobalFiler kit Mongolia
We are grateful to all the volunteers for providing DNA samples in Mongolia. Technical assistance for the DNA preparation and PCR amplification of the sample in this study by Sun Yoon, A-Young Sim, Ji-Eun Lee, and Eun-Bi Shin was also greatly appreciated. This research was supported by a fund (NFS2015DNA06/NFS2016DNA04) from the Forensic Research Program of the National Forensic Service, South Korea. The National Institute of Forensic Science, Ulaanbaatar, Mongolia supports Ganbold Suren. This work is also supported in part by DANKOOK ChemBio Specialization for the Creative Korea-II (2016).
Compliance with Ethical Standards
Conflict of interest
All authors declares that they have no conflict of interest.
The study was approved by the Ethics Committee and Institutional Review Board of Dankook University, Republic of Korea.
- Bacher JW, Helms C, Donis-Keller H, Hennes L, Nassif N, Schumm JW (2000) Chromosome localization of CODIS loci and new pentanucleotide repeat loci. In: Sensabaugh GF, Lincoln PJ, Olaisen B (eds) Progress in forensic genetics 8. Elsevier, New York, pp 33–36Google Scholar
- de Knijff P, Kayser M, Caglià A, Corach D, Fretwell N, Gehrig C, Graziosi G, Heidorn F, Herrmann S, Herzog B, Hidding M, Honda K, Jobling M, Krawczak M, Leim K, Meuser S, Meyer E, Oesterreich W, Pandya A, Parson W, Penacino G, Perez-Lezaun A, Piccinini A, Prinz M, Roewer L (1997) Chromosome Y microsatellites: population genetic and evolutionary aspects. Int J Legal Med 110:134–149CrossRefPubMedGoogle Scholar
- Felsenstein J (2013) PHYLIP version 3.695. Department of Genome Sciences, University of Washington, SeattleGoogle Scholar
- Hill CR, Duewer DL, Kline MC, Sprecher CJ, McLaren RS, Rabbach DR, Krenke BE, Ensenberger MG, Fulmer PM, Storts DR, Butler JM (2011) Concordance and population studies along with stutter and peak height ratio analysis for the PowerPlex® ESX 17 and ESI 17 systems. Forensic Sci Int Genet 5:269–275CrossRefPubMedGoogle Scholar
- Kayser M, Caglià A, Corach D, Fretwell N, Gehrig C, Graziosi G, Heidorn F, Herrmann S, Herzog B, Hidding M, Honda K, Jobling M, Krawczak M, Leim K, Meuser S, Meyer E, Oesterreich W, Pandya A, Parson W, Penacino G, Perez-Lezaun A, Piccinini A, Prinz M, Schmitt C, Roewer L (1997) Evaluation of Y-chromosomal STRs: a multicenter study. Int J Legal Med 110:125–133CrossRefPubMedGoogle Scholar
- Ramos-González B, Aguilar-Velázquez JA, de Lourdes Chávez-Briones M, Delgado-Chavarría JR, Alfaro-Lopez E, Rangel-Villalobos H (2016) Population data of 24 STRs in Mexican-Mestizo population from Monterrey, Nuevo Leon (Northeast, Mexico) based on PowerPlex® Fusion and GlobalFiler® kits. Forensic Sci Int Genet 21:e15–e17CrossRefPubMedGoogle Scholar
- Rerkamnuaychoke B, Rinthachai T, Shotivaranon J, Jomsawat U, Siriboonpiputtana T, Chaiatchanarat K, Pasomsub E, Chantratita W (2006) Thai population data on 15 tetrameric STR loci-D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818 and FGA. Forensic Sci Int 158:234–237CrossRefPubMedGoogle Scholar
- Schneider PM (2009) Expansion of the European standard set of DNA database loci—the current situation. Profiles DNA 12:6–7Google Scholar
- Sprecher C, Krenke B, Amiott B, Rabbach D, Grooms K (2000) The PowerPlex™ 16 System. Profiles DNA 4:3–6Google Scholar
- Tereba A (1999) Tools for analysis of population statistics. Profiles DNA 2:14–16Google Scholar