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Cooperation of erythrocytes with leukocytes in immune response of a teleost Oplegnathus fasciatus

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Abstract

The most abundant cell type in the blood of mammals and fish is erythrocyte. Unlike mammalian erythrocyte, fish erythrocyte is nucleated. The functional differentiation of teleost erythrocyte is insufficient compared with that of mammals. Therefore, fish erythrocyte may have different functions from that of mammals. Functional interaction between erythrocyte and leukocyte was confirmed by the cDNA microarray newly constructed in this study to investigate characterization of rock bream erythrocyte. In this study, different immune related genes of erythrocytes were annotated by microarray analysis. Microarray analysis showed that a total of 338 genes were up-regulated in co-cultured erythrocytes with leukocytes by LPS stimulation when comparing to erythrocytes stimulated LPS. Many genes in erythrocyte of rock bream were up-regulated in presence of leukocytes, suggesting that erythrocytes interact with leukocytes to trigger expression of various genes associated with the immune system. Our results provide valuable information that direct and indirect immunological function of fish erythrocyte.

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Acknowledgments

This research was a part of the project titled ‘Integrating technology development of quality control and ICT smart system in live fish for export (2016)’, funded by the Ministry of Oceans and Fisheries, Korea (Fishery Commercialization Technology Development Program) and a Grant from Marine Biotechnology Program (PJT200620, Genome Analysis of Marine Organisms and Development of Functional Applications) Funded by Ministry of Oceans and Fisheries, Korea. It was also supported by the project titled ‘Development of rapid and sensitive methods for assessing health in farmed fish (2016)’, funded by the Ministry of Oceans and Fisheries, Korea (Fishery Commercialization Technology Development Program). This research was a part of the project titled ‘Development of antimicrobial peptide for animal feed additives using marine worms (Polycheata) (20150284)’, funded by the Ministry of Oceans and Fisheries, Korea (Fishery Commercialization Technology Development Program).

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  1. Institute of Marine Industry, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-Gil, Tongyeong, Gyeongnam, 650-160, Republic of Korea

    Ji-Min Jeong, Mu-Chan Kim & Chan-Il Park

  2. Biotechnology Research Division, National Institute of Fisheries Science, 216 Gijanghaean-ro, Gijang-eup, Gijang-Gun, Busan, 619-705, Republic of Korea

    Cheul Min An

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  1. Ji-Min Jeong
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13258_2016_437_MOESM1_ESM.docx

Supplementary material 1. Summary of genes up-regulated in rock bream red blood cell. Differentially expressed genes were classified into broad functional categories according to gene ontology terms and the published literature (The unclassified group such as no significant similarly, unnamed protein and hypothetical protein are not included). Red color: up-regulated gene; green color: down-regulated gene; yellow color: no change. (DOCX 1021 kb)

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Jeong, JM., An, C.M., Kim, MC. et al. Cooperation of erythrocytes with leukocytes in immune response of a teleost Oplegnathus fasciatus . Genes Genom 38, 931–938 (2016). https://doi.org/10.1007/s13258-016-0437-z

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