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Cloning and expression analysis of UpPDS gene in Ulva pertusa

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Abstract

Phytoene desaturase (PDS) is an essential constituent of several carotenoid biosynthetic enzymes, which catalyzes phytoene into ζ-carotenoids. Here, a cDNA encoding PDS gene named UpPDS in Ulva pertusa was cloned by the approaches of expressed sequence tag sequencing of suppression subtractive hybridization cDNA library and rapid amplification of cDNA ends technique. The results showed that the full-length cDNA of UpPDS was 2029 bp, containing 1599 bp open reading frame and encoding 532 amino acids. Homology analysis showed that the deduced UpPDS protein was highly homologous to the PDS proteins from some algal species. Phylogenetic analysis indicated that the UpPDS was clustered together with PDS of Ectocarpus siliculosus firstly and was more close to PDS proteins from Chlamydomonas reinhardtii and Volvox carteri. The RT-qPCR analysis indicated that the transcript level of UpPDS was upregulated during the period of emersion.

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Abbreviations

PDS:

Phytoene desaturase

SSH:

Suppression subtractive hybridization

RACE:

Rapid amplification of cDNA ends

ORF:

Open reading frame

ABA:

Abscisic acid

PCR:

Polymerase chain reaction

RT qPCR:

Real time quantitative PCR

NCBI:

National center for biotechnology information

ESTs:

Expressed sequence tags

pI:

Isoelectric point

GMQE:

Global model quality estimation score

MUSCLE:

Multiple sequence comparison by log expectation

IPTG:

Isopropyl β-d-1-thiogalactopyranoside

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Authors and Affiliations

  1. College of Life Sciences, Liaoning Normal University and Key Laboratory of Plant Biotechnology of Liaoning Province, Dalian, 116081, People’s Republic of China

    Shaoming Tong, Haixiu Xi, Kejun Ai & Hesheng Hou

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  1. Shaoming Tong
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  2. Haixiu Xi
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  3. Kejun Ai
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  4. Hesheng Hou
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Correspondence to Hesheng Hou.

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Tong, S., Xi, H., Ai, K. et al. Cloning and expression analysis of UpPDS gene in Ulva pertusa . Genes Genom 38, 285–292 (2016). https://doi.org/10.1007/s13258-015-0365-3

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