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Comparison of transcriptome analysis between silk gland of B. mori and B. mandarina using next generation sequencing

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Abstract

Next-generation sequencing (NGS) technique applies to genome sequencing, genome resequencing, transcriptome profiling (RNA-Seq) and epigenome characterization. In this study, we built valuable genetic off-line databases related to silk synthesis of wild silkworm B. mandarina, using comparative transcriptional studies between B. mandarina and the domestic silkworm, on the NGS platform. It contains 12,298 coding DNA sequence (CDS) showing differential expressed in the silk gland of B. mandarina and B. mori. The other database contains 31 CDS that are estimated to be specifically expressed in the silk gland of B. mandarina.

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Acknowledgments

This work was supported by a grant (PJ009258012015) of the Agenda Program, Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology, RDA, Jeonju, 55365, Republic of Korea

    Yulan Piao, Seongwan Kim, Seongryul Kim & Kwangho Choi

  2. Department of Biochemistry, School of Medicine, Donguk University, Gyeongju, 38066, Republic of Korea

    Taewon Goo

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  1. Yulan Piao
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  2. Seongwan Kim
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  3. Seongryul Kim
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  4. Taewon Goo
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  5. Kwangho Choi
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Correspondence to Kwangho Choi.

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Piao, Y., Kim, S., Kim, S. et al. Comparison of transcriptome analysis between silk gland of B. mori and B. mandarina using next generation sequencing. Genes Genom 38, 251–262 (2016). https://doi.org/10.1007/s13258-015-0361-7

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  • DOI: https://doi.org/10.1007/s13258-015-0361-7

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