Gender-specific association of polymorphisms in the 5′-UTR and 3′-UTR of VEGF gene with hypertensive patients
Vascular endothelial growth factor (VEGF) is one of the most potent angiogenic factors. VEGF plasma levels have been reported to be significantly elevated in the patients with hypertension. This study aims to elucidate the associations of the VEGF −634G>C and 936C>T polymorphisms with the hypertensive patients in Korea. Genotyping of the VEGF polymorphisms was carried out by polymerase chain reaction and endonuclease digestion. The C allele of the VEGF −634G>C polymorphism and T allele of the 936C>T polymorphism were significantly higher in the hypertensive patients than in the normotensive controls. When the data of the genotype were stratified by gender, the association was gender-specific. We also found the associations of the haplotypes, composed of the VEGF −634G>C and 936C>T polymorphisms, with hypertensive group. Dominant models of −634G>C and 936C>T polymorphisms were associated with risk components of hypertension, according to the stratification analysis. Thus, this study demonstrates that the VEGF −634G>C and 936C>T polymorphisms may provide important genetic markers in identifying people at risk for hypertension. So far, association studies of VEGF gene polymorphisms with the hypertensive patients were rare. Therefore, further evaluation is required in other ethnic or racial populations with large samples to confirm our data.
KeywordsAssociation Gender-specific Haplotype Hypertension VEGF polymorphism
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (NRF-2012R1A1A4A01012216).
Conflict of interest
The authors declare that they have no conflict of interest.
Compliance of the bioethical standards
Written informed consent was approved by the Institutional Review Board of Jeju National University Hospital. The experimental procedures followed the standard regulation of the Review Board.
- Adams J, Carder PJ, Downey S, Forbes MA, MacLennan K, Allgar V, Kaufman S, Hallam S, Bicknell R, Walker JJ et al (2000) Vascular endothelial growth factor (VEGF) in breast cancer: comparison of plasma, serum, and tissue VEGF and microvessel density and effects of tamoxifen. Cancer Res 60:2898–2905PubMedGoogle Scholar
- Hamedian AA, Esteghamati A, Noshad S, Mozafari M, Moin-Tavakkoli H, Nakhjavani M, Mahmoudi T, Nikzamir M, Safary R, Nikzamir A (2012) Vascular endothelial growth factor (VEGF) +405 C/G polymorphism is associated with essential hypertension in a population from Tehran of Iran. Mol Biol Rep 39:6213–6218CrossRefPubMedGoogle Scholar
- Kim YR, Kim SH, Kang SH, Kim HJ, Kong MH, Hong S (2014) Association of the K173R variant and haplotypes in the aldosterone synthase gene with essential hypertension. Genes Genom 35:13–20Google Scholar
- Lambrechts D, Poesen K, Fernández-Santiago R, Al-Chalabi A, Del Bo R, Van Vught PW, Khan S, Marklund SL, Brockington A, van Marion I et al (2009) Meta-analysis of vascular endothelial growth factor variations in amyotrophic lateral sclerosis: increased susceptibility in male carriers of the -2578AA genotype. J Med Genet 46:840–846CrossRefPubMedGoogle Scholar